Western blot of human Jurkat (lanes 1 & 4), HUVEC (lanes 2 & 5), and A431 (lanes 3 & 6) cells. The blots were probed with anti-Vav2 (a.a. 309-322) at a dilution of 1:500 (lanes 1-3) and anti-Vav3 (a.a. 293-305) at 1:500 (lanes 4-6).
Bulk Order Anti-Vav2 Antibody
The Vav family of Rho-guanine nucleotide exchange factors, Vav1, Vav2, and Vav3, have central roles in transducing signals from cell surface receptors, such as integrin, growth factor and immune cell receptors to the cytoskeleton. This role includes receptor-mediated changes in the actin cytoskeleton and cell motility. Vav1 expression is normally restricted to hematopoietic cells, while Vav2 and Vav3 are more widely expressed. All three Vav isoforms have been shown to be abnormally expressed in several types of cancer. Vavs are composed of multiple domains, including a Dbl homology domain, a calponin homology domain, an acidic amino acid region, a pleckstrin homology domain, a cysteine-rich domain, and SH3 and SH2 domains. Vav activity is regulated by the phosphorylation status of several conserved tyrosine residues in the acidic region (In Vav2: Tyr-142, Tyr-159, and Tyr-172). These tyrosine residues are able to participate in autoinhibitory interactions with the Dbl homology domain and this interaction is prevented after phosphorylation of these sites leading to activation of Vav GEF activity.
Antigen Affinity Purified
A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the Dbl homology domain of human Vav2. This sequence has high homology with similar regions in rat and mouse Vav2, and has low homology to other Vav family members.
Chicken, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
The antibody detects a 100 kDa* doublet corresponding to the molecular mass of Vav2 on SDS-PAGE immunoblots of human Jurkat, HUVEC, and A431 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.