Anti-VASP (Thr-278), Phosphospecific Antibody

Our VASP (Thr-278) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat VASP (Thr-278) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot image of human A431 cells stimulated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal VASP (C-term.) antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4).
Immunocytochemical labeling of VASP phosphorylation in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal VASP (Thr-278) antibody, then detected using appropriate secondary antibodies conjugated to Cy3. The antibody was used in the absence (top left) or presence (top right) of blocking peptide (VX2785). Corresponding phase images are shown bottom left and right.
Western blot image of human A431 cells stimulated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal VASP (C-term.) antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4).
Western blot image of human A431 cells stimulated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal VASP (C-term.) antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4).
Immunocytochemical labeling of VASP phosphorylation in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal VASP (Thr-278) antibody, then detected using appropriate secondary antibodies conjugated to Cy3. The antibody was used in the absence (top left) or presence (top right) of blocking peptide (VX2785). Corresponding phase images are shown bottom left and right.
Human, Mouse, Rat
ICC, WB
Rabbit

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from PhosphoSolutions

SKU: VP2781

Volume: 100 µL
Price:
$329

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