Our Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, pig, and rat Ubiquitin C-terminal Hydrolase 1 (UCHL1) and is Protein G purified. It is great for use in WB, IHC, ICC.
Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.
Protein G Purified
ICC, IHC, WB
Recombinant full length human UCHL1 purified from E. coli.
Bovine, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Doran JF, Jackson P, Kynoch PA, Thompson RJ, 1983. Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis. Journal of Neurochemistry, 40:1542-7. PMID: 6343558
Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J, 1989. The neuronspecific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase. Science, 246:670-3. PMID: 2530630
Kurihara LJ, Kikuchi T, Wada K, Tilghman SM, 2001. Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia. Human Molecular Genetics, 10:1963-70. PMID: 11555633
Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr, 2002. The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Cell 111:209-18. PMID: 12408865
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