Name: Anti-Tryptophan Hydroxylase Antibody
Brand: PhosphoSolutions
SKU: 2015-TPH
Price: 415.0 USD
Availability: InStock

Our Anti-Tryptophan Hydroxylase sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Tryptophan Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Human, Rat

Expected Reactivity

Bovine, Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Mouse, Non-Human Primate, Sheep, Vole

Applications

IHC, WB

Host Species

Sheep

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

TPH1

Molecular Weight

55 kDa

Go to Full Product Details

Western blot of human dorsal Raphe nucleus showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Tryptophan Hydroxylase Antibody

Product Details

Target

Tryptophan Hydroxylase

Target Description

Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Sheep

Gene Name

TPH1

Molecular Weight

55 kDa

Antigen

Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Species Reactivity

Human, Rat

Expected Reactivity

Bovine, Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Mouse, Non-Human Primate, Sheep, Vole

Antibody Registry ID

AB_2492271

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled sheep serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Indoleacetic acid 5 hydroxylase antibody, L tryptophan hydroxylase antibody, MGC119994 antibody, TPH 1 antibody, TPH antibody, TPH1 antibody, TPH1_HUMAN antibody, TPRH antibody, TRPH antibody, Tryptophan 5 hydroxylase 1 antibody, Tryptophan 5 monooxygenase 1 antibody, Tryptophan 5 monooxygenase antibody, Tryptophan 5-hydroxylase 1 antibody, Tryptophan 5-monooxygenase 1 antibody, Tryptophan hydroxylase 1 antibody

UniProt Details

UniProt (Human): P17752
UniProt (Immunogen Species): P17290

NCBI Gene ID

100101558

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
11856572	0.5-1.0ug/ mL	Haycock, JW., et al. 2002. A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope. Journal of Neuroscience Methods, 114(2), pp.205-212.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Tryptophan Hydroxylase Antibody

Our Anti-Tryptophan Hydroxylase sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Tryptophan Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.