Our Anti-Tryptophan Hydroxylase sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Tryptophan Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Western blot of human dorsal Raphe nucleus showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein.
Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).
Antigen Affinity Purified from Pooled Serum
Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.