Our Anti-Troponin I (cardiac) Ser43 rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Troponin I (cardiac) Ser43 and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser43 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is greatly decreased by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
Anti-Troponin I (Cardiac) Ser43 Antibody
Bulk Order Anti-Troponin I (Cardiac) Ser43 Antibody
Troponin I (cardiac) Ser43
Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser-43 and Ser-45 of cardiac troponin I plays a substantial role in the PKC mediated depression. (Montgomery et al, 2002).
Antigen Affinity Purified from Pooled Serum
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 of mouse troponin I, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH).