Anti-Troponin I (Cardiac) Ser23/24 Antibody

Name: Anti-Troponin I (Cardiac) Ser23/24 Antibody
Brand: PhosphoSolutions
SKU: p2010-2324
Price: 430.0 USD
Availability: InStock

4 Citations

Our Anti-Troponin I (cardiac) Ser23/24 rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Troponin I (cardiac) Ser23/24 and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blots showing specific labeling of S23/S24 TnI, cat. p2010-2324 (upper) and TnI; cat. 2010-TnI (lower) in protein lysates of mouse cardiomyocytes. Image from publication CC-BY-4.0. PMID: 36424917

Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser23/24 in the second lane (-). Phosphospecificity is shown in the first lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Species Reactivity

Mouse, Rat

Applications

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p2010-2324

Volume: 100 µL

Variant

Price:

$430

Quantity:

Product Details

Target

Troponin I (cardiac) Ser23/24

Target Description

Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the calcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by extension enhances rates of cross-bridge cycling and diastolic relaxation (Noland, Jr. et al., 1995; Noland et al., 1989). In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosphorylation of TnI (largely at Ser-43) inhibits the actin-cross bridge reaction and reduces the Ca++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation (Noland, Jr. and Kuo, 1991). Phosphorylation at Thr-144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates (Sumandea et al., 2008). Importantly, changes in the phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression (Walker et al., 2010).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

TNNI3

Molecular Weight

25 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser23/24 of mouse troponin 1, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Non-Human Primate

Antibody Registry ID

AB_2492268

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~25 kDa cardiac troponin I protein phosphorylated at Ser23/24. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser23,24

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

cardiac muscle antibody, Cardiac troponin I antibody, Cardiac Troponin I antibody, cardiomyopathy dilated 2A (autosomal recessive) antibody, Cardiomyopathy familial hypertrophic 7 included antibody, CMD1FF antibody, CMD2A antibody, CMH7 antibody, cTnI antibody, Familial hypertrophic cardiomyopathy 7 antibody, MGC116817 antibody, RCM1 antibody, Tn1 antibody, Tni antibody, TNN I3 antibody, TNNC 1 antibody, TNNC1 antibody, TNNI3 antibody, TNNI3_HUMAN antibody, Troponin I antibody, Troponin I cardiac antibody, Troponin I cardiac muscle antibody, Troponin I cardiac muscle isoform antibody, Troponin I type 3 cardiac antibody, troponin I cardiac 3 antibody, TroponinI antibody, Troponin I type 3 (cardiac) antibody

UniProt Details

UniProt (Human): P19429
UniProt (Immunogen Species): P48787

NCBI Gene ID

21954

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
24291343	1:1000	Thoemmes, S.F., et al. 2014. Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of Immunological Methods, 403(1-2), pp.66-71.
23564307	1:1000	Walker, L.A., et al. 2013. Contractile protein phosphorylation predicts human heart disease phenotypes. American Journal of Physiology-Heart and Circulatory Physiology, 304(12), pp.H1644-H1650.

Western Blot: Mouse
PMID	Dilution	Publication
36424916	1:1000	Papa, A., et al. 2022. Rad Regulation Of CaV1.2 Channels Controls Cardiac Fight-Or-Flight Response. Nature Cardiovascular Research, 1022-1038.
24291343	1:1000	Thoemmes, S.F., et al. 2014. Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of Immunological Methods, 403(1-2), pp.66-71.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Troponin I (Cardiac) Ser23/24 Antibody

SKU: p2010-2324

Product Details

Product Specific References for Applications and Species

Product Specific Protocols

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