Name: Anti-Troponin I (cardiac) Antibody
Brand: PhosphoSolutions
SKU: 2010-TNI
Price: 360.0 USD
Availability: InStock

Our Anti-Troponin I (cardiac) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Troponin I (cardiac) and is neat serum. It is great for use in WB and IHC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Applications

IHC, WB

Host Species

Rabbit

Format

Neat Serum

Gene Name

TNNI3

Molecular Weight

25 kDa

Go to Full Product Details

Western blots showing specific labeling of S23/S24 TnI, cat. p2010-2324 (upper) and TnI; cat. 2010-TnI (lower) in protein lysates of mouse cardiomyocytes. Image from publication CC-BY-4.0. PMID: 36424916

Examination of cardiac troponin I (cTnI) (cat. 2010-TNI, green) and active β-Catenin (red) levels in embryos at E9.5 by immunofluorescence (IF) analysis. Pregnant mice were treated daily with vehicle or the JAKi (10 mg/kg body weight/day, i.p.) beginning with day 6.5 post-conception. Embryos were harvested at E9.5 for IF analysis. Image from publication. CC-BY-4.0

Western blot of 20 ug of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein.

Volume: 100 µL

Variant

Price:

$360

Quantity:

Bulk Order Anti-Troponin I (cardiac) Antibody

Product Details

Target

Troponin I (cardiac)

Target Description

Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the calcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by extension enhances rates of cross-bridge cycling and diastolic relaxation (Noland, Jr. et al., 1995; Noland et al., 1989). In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosphorylation of TnI (largely at Ser-43) inhibits the actin-cross bridge reaction and reduces the Ca++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation (Noland, Jr. and Kuo, 1991). Phosphorylation at Thr-144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates (Sumandea et al., 2008). Importantly, changes in the phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression (Walker et al., 2010).

Format

Neat Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

TNNI3

Molecular Weight

25 kDa

Antigen

Fusion protein of complete mouse cardiac troponin 1 holoprotein.

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2492267

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Neat Serum

Buffer

Neat serum

Dilution Ranges

WB: 1:2000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~25 kDa cardiac troponin I protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

cardiac muscle antibody, Cardiac troponin I antibody, Cardiac Troponin I antibody, cardiomyopathy dilated 2A (autosomal recessive) antibody, Cardiomyopathy familial hypertrophic 7 included antibody, CMD1FF antibody, CMD2A antibody, CMH7 antibody, cTnI antibody, Familial hypertrophic cardiomyopathy 7 antibody, MGC116817 antibody, RCM1 antibody, Tn1 antibody, Tni antibody, TNN I3 antibody, TNNC 1 antibody, TNNC1 antibody, TNNI3 antibody, TNNI3_HUMAN antibody, Troponin I antibody, Troponin I cardiac antibody, Troponin I cardiac muscle antibody, Troponin I cardiac muscle isoform antibody, Troponin I type 3 cardiac antibody, troponin I cardiac 3 antibody, TroponinI antibody, Troponin I type 3 (cardiac) antibody

UniProt Details

UniProt (Immunogen Species): P48787

NCBI Gene ID

21954

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Human | Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
37360690	1:200	Chi, C, et al. 2023. Interferon Hyperactivity Impairs Cardiogenesis in Down Syndrome via Downregulation of Canonical Wnt Signaling. iScience, 107012.

Western Blot: Human
PMID	Dilution	Publication
24291343	1:5000	Thoemmes, S.F., et al. 2014. Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of Immunological Methods, 403(1-2), pp.66-71.
23564307	1:2500	Walker, L.A., et al. 2013. Contractile protein phosphorylation predicts human heart disease phenotypes. American Journal of Physiology-Heart and Circulatory Physiology, 304(12), pp.H1644-H1650.

Western Blot: Mouse
PMID	Dilution	Publication
36802963	not listed	Yusifova, M., et al. 2023. Voluntary Wheel Running Exercise Does Not Attenuate Circadian and Cardiac Dysfunction Caused by Conditional Deletion of Bmal1. Journal of Biological Rhythms, 7487304231152398.
36424916	1:2000	Papa, A., et al. 2022. Rad Regulation Of CaV1.2 Channels Controls Cardiac Fight-Or-Flight Response. Nature Cardiovascular Research, 1022-1038.
24291343	1:5000	Thoemmes, S.F., et al. 2014. Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of Immunological Methods, 403(1-2), pp.66-71.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Troponin I (cardiac) Antibody

Our Anti-Troponin I (cardiac) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Troponin I (cardiac) and is neat serum. It is great for use in WB and IHC.