Western blot of mouse spleen (lanes 1-4) and human K562 cells (lanes 5-8). The blots were probed with anti-themis (N-terminal region) rabbit polyclonal antibody at 1:2000 (lanes 1 & 5), 1:1000 (lanes 2 & 6), 1:500 (lanes 3 & 7), or 1:500 in the presence of themis blocking peptide (TX3885) (lanes 4 & 8).
Anti-Themis (N-terminal region) Antibody
Bulk Order Anti-Themis (N-terminal region) Antibody
Themis (N-terminal region)
During positive selection, thymocytes transition through a stage during which T cell antigen receptor (TCR) signaling controls CD4-versus-CD8 lineage selection and maturation. Thymocyte-expressed molecule involved in selection (themis) is critically required for this thymocyte selection. Themis has been identified as several molecules: Grb2-associating protein (GASP), signaling phosphoprotein specific for T cells (SPoT), TSEPA, and thylex. Themis is a cytosolic protein with no known functional motifs. Its expression is observed primarily in T cells, especially immature CD4/CD8 double positive thymocytes. Themis-deficient thymocytes have defective positive selection, and fewer numbers of mature thymocytes. These themis-induced effects may be due to defective TCR signaling since themis constitutively associates with the TCR adaptor Grb2, and themis is phosphorylated quickly after TCR stimulation. In addition, themis is required for optimal TCR-driven calcium mobilization and activation of the MAP kinase Erk. Thus, themis may be an important adaptor protein in TCR signaling.
Antigen Affinity Purified
Themis (N-terminal region) antibody was generated from a peptide that included amino acids in the N-terminal region of human themis. This sequence has high homology to the conserved region in rat and mouse themis.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
The antibody detects a 72 kDa* protein corresponding to the molecular mass of themis on SDS-PAGE immunoblots of mouse spleen and human K562 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.