Anti-Themis (N-terminal region) Antibody

Name: Anti-Themis (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: TP3881
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

THEMIS

Molecular Weight

Go to Full Product Details

Western blot of mouse spleen (lanes 1-4) and human K562 cells (lanes 5-8). The blots were probed with anti-themis (N-terminal region) rabbit polyclonal antibody at 1:2000 (lanes 1 & 5), 1:1000 (lanes 2 & 6), 1:500 (lanes 3 & 7), or 1:500 in the presence of themis blocking peptide (TX3885) (lanes 4 & 8).

Volume: 100 µL

Variant

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$255

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Bulk Order Anti-Themis (N-terminal region) Antibody

Product Details

Target

Themis (N-terminal region)

Target Description

During positive selection, thymocytes transition through a stage during which T cell antigen receptor (TCR) signaling controls CD4-versus-CD8 lineage selection and maturation. Thymocyte-expressed molecule involved in selection (themis) is critically required for this thymocyte selection. Themis has been identified as several molecules: Grb2-associating protein (GASP), signaling phosphoprotein specific for T cells (SPoT), TSEPA, and thylex. Themis is a cytosolic protein with no known functional motifs. Its expression is observed primarily in T cells, especially immature CD4/CD8 double positive thymocytes. Themis-deficient thymocytes have defective positive selection, and fewer numbers of mature thymocytes. These themis-induced effects may be due to defective TCR signaling since themis constitutively associates with the TCR adaptor Grb2, and themis is phosphorylated quickly after TCR stimulation. In addition, themis is required for optimal TCR-driven calcium mobilization and activation of the MAP kinase Erk. Thus, themis may be an important adaptor protein in TCR signaling.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

THEMIS

Molecular Weight

Antigen

Themis (N-terminal region) antibody was generated from a peptide that included amino acids in the N-terminal region of human themis. This sequence has high homology to the conserved region in rat and mouse themis.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 72 kDa* protein corresponding to the molecular mass of themis on SDS-PAGE immunoblots of mouse spleen and human K562 cells.

Post Translational Modification Residue

203-216

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Gasp, SPoT, TSEPA, thylex

UniProt Details

UniProt (Human): Q8N1K5
UniProt (Immunogen Species): Q8N1K5

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Anti-Themis (N-terminal region) Antibody