Our Anti-TFAM (Transcription Factor A, mitochondrial) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human TFAM (Transcription Factor A, motochondrial) and is neat serum. It is great for use in WB.
TFAM (Transcription Factor A, motochondrial)
Mitochondrial Transcription Factor A (TFAM) is a key activator of mitochondrial (mt) DNA transcription as well as a participant in mitochondrial genome replication. mtDNA is highly susceptible to oxidative stress leading to mitochondrial dysfunction. Overexpression of TFAM has been implicated in the amelioration of age dependent impairment of brain functions through the prevention of oxidative stress and mitochondrial dysfunction in microglia (Hayashi et al., 2008). More recently, TFAM overexpression has been shown to potentially reduce oxidative stress in motor neurons and delay onset of amyotrophic lateral sclerosis (ALS) in ALS model mice (Morimoto et al., 2012).
Native recombinant human TFAM protein with c-terminal 6-his tag.
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Specific for endogenous levels of the ~24 kDa TFAM protein. The antibody has been directly tested for reactivity in human. Shows lesser reactivity to rat and mouse due to some sequence homology, but our catalog # 2001-TFAM is recommended for use in rodent.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Transcription factor 6-like 1 antibody, Mitochondrial transcription factor 1 antibody, mitochondrial transcription factor A antibody, MtTF1 antibody, mtTFA antibody, TCF 6 antibody, TCF-6 antibody, TCF6 antibody, TCF6L1 antibody, TCF6L2 antibody, TCF6L3 antibody, TFAM antibody, TFAM_HUMAN antibody, Transcription factor 6 antibody, Transcription factor 6 like 2 (mitochondrial transcription factor) antibody, Transcription factor 6 like 2 antibody, Transcription factor 6-like 2 antibody, transcription factor 6-like 3 antibody, Transcription factor A mitochondrial antibody, Transcription factor A mitochondrial antibody, Transcription factor A mitochondrial precursor antibody
UniProt (Human): Q00059
UniProt (Immunogen Species): Q00059
UniProt (Immunogen Species): Q00059
Product Specific References for Applications and Species
- Western Blot: Mouse
|Western Blot: Mouse|
|35487893||1:1000||Sidarala, V., et al. 2022. Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis. Nature Communications, 13(1), pp.1-16.|
- Hayashi, Y., Yoshida, M., Yamato, M., Ide, T., Wu, Z., Ochi-Shindou, M., Kanki, T., Kang, D., Sunagawa, K., Tsutsui, H. and Nakanishi, H., 2008. Reverse of age-dependent memory impairment and mitochondrial DNA damage in microglia by an overexpression of human mitochondrial transcription factor a in mice. Journal of Neuroscience, 28(34), pp.8624-8634. PMID: 18716221
- Morimoto, N., Miyazaki, K., Kurata, T., Ikeda, Y., Matsuura, T., Kang, D., Ide, T. and Abe, K., 2012. Effect of mitochondrial transcription factor a overexpression on motor neurons in amyotrophic lateral sclerosis model mice. Journal of Neuroscience Research, 90(6), pp.1200-1208. PMID: 22354563