Our Anti-TAO2 (Ser181) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat TAO2 (Ser181) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat cortical lysate showing specific immunolabeling of the ~120 kDa TAO2 phosphorylated at Ser181 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
Anti-TAO2 (Ser181) Antibody
In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999; Chen and Cobb, 2001). This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs (Chen et al., 2003). Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser-181 is located within this domain.
Antigen Affinity Purified from Pooled Serum
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser181 of human TAO2, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB Brain: 1:1000
Specific for endogenous levels of the ~120 kDa TAO2 phosphorylated at Ser181. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
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Chen, Z., Raman, M., Chen, L., Lee, S.F., Gilman, A.G. and Cobb, M.H., 2003. TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors. Journal of Biological Chemistry, 278(25), pp.22278-22283. PMID: 12665513
Chen, Z. and Cobb, M.H., 2001. Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2. Journal of Biological Chemistry, 276(19), pp.16070-16075. PMID: 11279118
Chen, Z., Hutchison, M. and Cobb, M.H., 1999. Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain. Journal of Biological Chemistry, 274(40), pp.28803-28807. PMID: 10497253
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