Name: Anti-Synaptotagmin (Thr202) Antibody
Brand: PhosphoSolutions
SKU: p1570-202
Price: 415.0 USD
Availability: InStock

Our Anti-Synaptotagmin (Thr202) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Synaptotagmin (Thr202) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Mouse, Zebrafish

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SYT1

Molecular Weight

62 kDa

Go to Full Product Details

Western blot of rat cortical lysate showing specific immunolabeling of the ~62 kDa synaptotagmin phosphorylated at Thr202 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Immunostaining of 14 DIV rat cortical neurons labeling synaptotagmin when phosphorylated at Thr202 (cat. p1570-202, 1:400, red) and PSD95 (green). The cortical neurons were fixed with methanol at -20C for 10 minutes. Photo courtesy of Gang Liu.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Synaptotagmin (Thr202) Antibody

Product Details

Target

Synaptotagmin (Thr202)

Target Description

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

SYT1

Molecular Weight

62 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Mouse, Zebrafish

Antibody Registry ID

AB_2492252

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:400

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Thr 202. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr202

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody

UniProt Details

UniProt (Human): P21579
UniProt (Immunogen Species): P21707

NCBI Gene ID

25716

Product Specific Protocols

Western Blot Protocol: Download

General References

Fernandez-Chacon, R., Königstorfer, A., Gerber, S.H., García, J., Matos, M.F., Stevens, C.F., Brose, N., Rizo, J., Rosenmund, C. and Südhof, T.C., 2001. Synaptotagmin I functions as a calcium regulator of release probability. Nature, 410(6824), p.41-49. PMID: 11242035
Hilfiker, S., Pieribone, V.A., Nordstedt, C., Greengard, P. and Czernik, A.J., 1999. Regulation of synaptotagmin I phosphorylation by multiple protein kinases. Journal of Neurochemistry, 73(3), pp.921-932. PMID: 10461881
Lee, B.H., Min, X., Heise, C.J., Xu, B.E., Chen, S., Shu, H., Luby-Phelps, K., Goldsmith, E.J. and Cobb, M.H., 2004. WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding. Molecular Cell, 15(5), pp.741-751. PMID: 15350218
Poskanzer, K.E., Marek, K.W., Sweeney, S.T. and Davis, G.W., 2003. Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature, 426(6966), p.559-563. PMID: 14634669
Wang, C.T., Lu, J.C., Bai, J., Chang, P.Y., Martin, T.F., Chapman, E.R. and Jackson, M.B., 2003. Different domains of synaptotagmin control the choice between kiss-and-run and full fusion. Nature, 424(6951), p.943-947. PMID: 12931189

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Anti-Synaptotagmin (Thr202) Antibody

Our Anti-Synaptotagmin (Thr202) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Synaptotagmin (Thr202) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.