Name: Anti-Synaptotagmin 1 Antibody
Brand: PhosphoSolutions
SKU: 1975-STG
Price: 415.0 USD
Availability: InStock

Our Anti-synaptotagmin 1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat synaptotagmin 1 and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Chicken, Human, Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SYT1

Molecular Weight

65 kDa

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Western blot of rat cortical lysate showing specific labeling of the ~65 kDa synaptotagmin protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Synaptotagmin 1 Antibody

Product Details

Target

Synaptotagmin 1

Target Description

Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

SYT1

Molecular Weight

65 kDa

Antigen

Synthetic peptide corresponding to amino acid residues specific to rat synaptotagmin 1, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Chicken, Human, Non-Human Primate

Antibody Registry ID

AB_2492251

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~65 kDa synaptotagmin I protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody

UniProt Details

UniProt (Human): P21579
UniProt (Immunogen Species): P21707

NCBI Gene ID

25716

Product Specific References for Applications and Species

Immunohistochemistry: Drosophila
Western Blot: Drosophila | Mouse

Immunohistochemistry: Drosophila
PMID	Dilution	Publication
36048753	1:100	Krzystek, T.J., et al. 2022. HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury. Autophagy, 1-22.

Western Blot: Drosophila
PMID	Dilution	Publication
36048753	1:1000	Krzystek, T.J., et al. 2022. HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury. Autophagy, 1-22.

Western Blot: Mouse
PMID	Dilution	Publication
32611447	1:1000	White, J.A., et al. 2020. . Excess Rab4 rescues synaptic and behavioral dysfunction caused by defective HTT-Rab4 axonal transport in Huntington’s disease. Acta Neuropathologica Communications, 8(1), pp.1-22.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Synaptotagmin 1 Antibody

Our Anti-synaptotagmin 1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat synaptotagmin 1 and is antigen affinity purified from pooled serum. It is great for use in WB.