Our Anti-synaptotagmin 1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat synaptotagmin 1 and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat cortical lysate showing specific labeling of the ~65 kDa synaptotagmin protein.
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
SYT1
65 kDa
Synthetic peptide corresponding to amino acid residues specific to rat synaptotagmin 1, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Chicken, Human, Non-Human Primate
AB_2492251
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~65 kDa synaptotagmin I protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Krzystek, T.J., et al. 2022. HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury. Autophagy, 1-22.
Krzystek, T.J., et al. 2022. HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury. Autophagy, 1-22.
