Western blot of rat hippocampal lysate showing specific immunolabeling of the ~145 kDa synaptojanin I protein.
Anti-Synaptojanin 1 Antibody
Synaptojanin is a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms; one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues (Ramjaun AR & McPherson PS, 1996). The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down’s syndrome (DS). It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidylinositol-4,5- bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice (Voronov SV et al., 2008).
Protein G Purified
Recombinant protein from the C-terminal region of rat synaptojanin 1.
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Protein G purified culture supernatant.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB Brain: 1:1000
Specific for endogenous levels of the ~145 kDa synaptojanin I protein in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Ramjaun, A.R. and McPherson, P.S., 1996. Tissue-specific alternative splicing generates two synaptojanin isoforms with differential membrane binding properties. Journal of Biological Chemistry, 271(40), pp.24856-24861. PMID: 8798761
Voronov, S.V., Frere, S.G., Giovedi, S., Pollina, E.A., Borel, C., Zhang, H., Schmidt, C., Akeson, E.C., Wenk, M.R., Cimasoni, L. and Arancio, O., 2008. Synaptojanin 1-linked phosphoinositide dyshomeostasis and cognitive deficits in mouse models of Down's syndrome.Proceedings of the National Academy of Sciences, 105(27), pp.9415-9420. PMID: 18591654
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