Our Anti-Synapsin (Ser62,67) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Synapsin (Ser62,67) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat cortical lysate showing specific labeling of the ~78 kDa synapsin protein phosphorylated at Ser62,67 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser-549 along with Ser-62 and Ser-67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987; Jovanovic et al., 1996).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
SYN1
78 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser62/67 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH).
Rat
Bovine
AB_2492245
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser62,67. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser62,67
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody
Tallent, M.K., et al. 2009. In Vivo Modulation of O-GlcNAc Levels Regulates Hippocampal Synaptic Plasticity through Interplay with Phosphorylation. Journal of Biological Chemistry, Jan 2009; 284: 174 – 181.
Chanaday, N.L., et al. 2015. Glutamate Release Machinery Is Altered in the Frontal Cortex of Rats with Experimental Autoimmune Encephalomyelitis. Molecular Neurobiology, 51(3), 1353-1367.
