Anti-Synapsin (Ser549) Antibody

Name: Anti-Synapsin (Ser549) Antibody
Brand: PhosphoSolutions
SKU: p1560-549
Price: 415.0 USD
Availability: InStock

from PhosphoSolutions Datasheet Trial Size Pooled Serum

Our Anti-Synapsin (Ser549) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Synapsin (Ser549) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SYN1

Molecular Weight

78 kDa

Go to Full Product Details

Immunostaining of cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser549(cat. p1560-549, green, 1:500). Cells and photo courtesy of QBMCellScience.

Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser549 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Synapsin (Ser549) Antibody

Product Details

Target

Synapsin (Ser549)

Target Description

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser-549 along with Ser-62 and Ser-67 are the sites of synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996). Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

SYN1

Molecular Weight

78 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate

Antibody Registry ID

AB_2492246

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser549. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser549

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody

UniProt Details

UniProt (Human): P17600
UniProt (Immunogen Species): P17599

NCBI Gene ID

281510

General References

Jovanovic JN, Benfenati, F, Siow YL, Sihra TS, Sanghera JS, Pelech SL, Greengard P, Czernik AJ (1996) Neurotrophins stimulate phosphorylation of Synapsin I by MAP kinase and regulate Synapsin I-actin interactions. Neurobiology 93:3679-3683. PMID: 8622996
Czernik AJ, Pang DT, Greengard P (1987) Amino acid sequences surrounding the cAMP-dependent and calcium/calmodulin-dependent phosphorylation sites in rat and bovine synapsin I. PNAS (USA) 84:7518-7522. PMID: 3118371
Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. Journal of Neuroscience 22:4372- 4380.PMID: 12040043
Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. Journal of Neuroscience 21:7944-7953. PMID: 11588168
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neuroscience 5:431-437.PMID: 11976703
Nayak AS, Moore CI, Browning MD** (1996) CaM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. PNAS (USA) 93:15451-15456. PMID: 8986832
**Dr. Michael Browning co-author of the cited paper above is the President and founder of PhosphoSoluti

Recover password

Create my account

Anti-Synapsin (Ser549) Antibody

Our Anti-Synapsin (Ser549) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Synapsin (Ser549) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.