Name: Anti-Synapsin I (Ser603) Antibody
Brand: PhosphoSolutions
SKU: p1560-603
Price: 415.0 USD
Availability: InStock

Our Anti-Synapsin I (Ser603) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Synapsin I (Ser603) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Xenopus, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SYN1

Molecular Weight

78 kDa

Go to Full Product Details

Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Synapsin I (Ser603) Antibody

Product Details

Target

Synapsin I (Ser603)

Target Description

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine 603 is the site on synapsin I that is phosphorylated by calcium calmodulin kinase II and by p21-activated kinases (Sakurada et al., 2002; Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function (Nayak et al., 1996; Bahler and Greengard, 1987; McGuinness et al., 1989).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

SYN1

Molecular Weight

78 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser603 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Xenopus, Zebrafish

Antibody Registry ID

AB_2492247

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser603 . Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser603

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody

UniProt Details

UniProt (Human): P17600
UniProt (Immunogen Species): P17599

NCBI Gene ID

281510

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat

Western Blot: Human
PMID	Dilution	Publication
23255606	1:10,000	Magupalli, V.G., et al. 2013. Ca²⁺-independent activation of Ca²⁺/calmodulin-dependent protein kinase II bound to the C-terminal domain of Ca_V2.1 calcium channels. Journal of Biological Chemistry, 288(7), 4637-4648.

Western Blot: Mouse
PMID	Dilution	Publication
34206873	1:1000	Nitta, A., et al. 2021. Schizophrenia-like behavioral impairments in mice with suppressed expression of piccolo in the medial prefrontal cortex. Journal of Personalized Medicine, 11(7), p.607.
19609731	1:1000	Takahashi, E., et al. 2010. Interaction Between Ca_V2.1 α 1 and CaMKII in Ca_V2.1 α 1 Mutant Mice, Rolling Nagoya. Journal of Molecular Neuroscience, 41(2), pp.223-229.

Western Blot: Rat
PMID	Dilution	Publication
18519737	not listed	Leal-Ortiz, S., et al. 2008. Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. Journal of Cell Biology, 181: 831 - 846.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Synapsin I (Ser603) Antibody

Our Anti-Synapsin I (Ser603) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Synapsin I (Ser603) and is antigen affinity purified from pooled serum. It is great for use in WB.