Name: Anti-Synapsin I (100 µL) Antibody
Brand: PhosphoSolutions
SKU: 1926-SYNP
Price: 415.0 USD
Availability: InStock

Our Anti-Synapsin I rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Synapsin I and is antigen affinity purified from pooled serum. It is great for use in WB, ICC, IP.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SYN1

Molecular Weight

78 kDa

Go to Full Product Details

Immunostaining of 40DIV cultured rat cortical neurons showing punctate labeling of synapsin (cat. 1926-SYNP, 1:1000, red). The blue is staining nuclear DNA. Cells and photo courtesy of QBMCellScience.

Immunostaining of cultured mouse caudate neurons showing punctate distribution of synapsin (cat. 1926-SYNP, 1:1000, green) and MAP (red). Cells and photo courtesy of QBMCellScience.

Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Synapsin I (100 µL) Antibody

Product Details

Target

Synapsin I

Target Description

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, IP, WB

Host Species

Rabbit

Gene Name

SYN1

Molecular Weight

78 kDa

Antigen

Native protein purified from bovine brain.

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492242

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the native protein was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:1000-1:2000
IP: 1 µl per 200 µg lysate

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody

UniProt Details

UniProt (Human): P17600
UniProt (Immunogen Species): P17599

NCBI Gene ID

281510

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
26706461	1:2000	Wahba, G., et al. 2016. A rapid and efficient method for dissociated cultures of mouse myenteric neurons. Journal of Neuroscience Methods, 261, pp.110-116.

Western Blot: Human
PMID	Dilution	Publication
23255606	1:10,000	Magupalli, V.G., et al. 2013. Ca²⁺-independent activation of Ca²⁺/calmodulin-dependent protein kinase II bound to the C-terminal domain of Ca_V2.1 calcium channels. Journal of Biological Chemistry, 288(7), pp.4637-4648.

Western Blot: Mouse
PMID	Dilution	Publication
27335405	not listed	Song, S.H., et al. 2016. Synapsin isoforms regulating GABA release from hippocampal interneurons. Journal of Neuroscience, 36(25), pp.6742-6757.

Western Blot: Rat
PMID	Dilution	Publication
18819923	1:1000	Davies, K.D., et al. 2008. Long term synaptic depression that is associated with GluR1 dephosphorylation but not α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization. Journal of Biological Chemistry, 283(48), pp.33138-33146.
18819923	not listed	Grosshans, D.R., et al. 2002. LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nature Neuroscience, Jan;5(1):27-33.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Synapsin I (100 µL) Antibody

Our Anti-Synapsin I rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Synapsin I and is antigen affinity purified from pooled serum. It is great for use in WB, ICC, IP.