Anti-Sphingosine Kinase 2 (N-terminal region) Antibody

Name: Anti-Sphingosine Kinase 2 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: SP4621
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

7 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

SPHK2

Molecular Weight

Go to Full Product Details

Western blot of human recombinant SK2 (lanes 1-4) and HeLa treated with calyculin A (lanes 5-8). The blots were untreated (lane 1, 3, 5 & 7) or treated with lambda phosphatase (lane 2, 4, 6 & 8), then probed with anti-SK2 (N-terminal region) (lanes 1, 2, 5 & 6) or anti-SK2 (Thr-578) (lanes 3, 4, 7, & 8).

Volume: 100 µL

Variant

Price:

$309

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Product Details

Target

Sphingosine Kinase 2 (N-terminal region)

Target Description

Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. Regulation of SK1 and SK2 activity may occur through phosphorylation. SK1 is phosphorylated at Ser-225 by ERK leading to increased activity and translocation to the plasma membrane. SK2 is phosphorylated in response to EGF, PKC activators, and phorbol esters. ERK1 can phosphorylate both Ser-351 and Thr-578, and non-phosphorylatable mutants of these sites suppress ERK1-mediated chemotaxis.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IP, WB

Host Species

Rabbit

Gene Name

SPHK2

Molecular Weight

Antigen

SK2 (N-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human SK2. This peptide sequence is highly conserved in rat and mouse SK2 proteins, and has no homology to SK1.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:250
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects 70 kDa* proteins corresponding to the molecular mass of SK2 on SDS-PAGE immunoblots of human recombinant SK2 and endogenous SK2 in human HeLa cells. The antibody also works for ELISA, immunoprecipitation, and immunocytochemistry.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SK2, Spk2, Sphk2

UniProt Details

UniProt (Human): Q9NRA0
UniProt (Immunogen Species): Q9NRA0

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
30250299	1:300	Neubauer, HA, et al. 2019. Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma. Oncogene, 1151-1165.

Western Blot: Human
PMID	Dilution	Publication
37389926	not listed	Seidita, I, et al. 2023. Sphingosine 1-phosphate elicits a ROS-mediated proinflammatory response in human endometrial stromal cells via ERK5 activation. The FASEB Journal, e23061.
33525436	not listed	Bernacchioni, C, et al. 2021. Role of Sphingosine 1-Phosphate Signalling Axis in Muscle Atrophy Induced by TNFα in C2C12 Myotubes. International journal of molecular sciences, 1280.
33500141	not listed	Bernacchioni, C, et al. 2021. Sphingosine 1-phosphate signaling in uterine fibroids: implication in activin A pro-fibrotic effect. Fertility and Sterility, 1576-1585.
31477835	not listed	Bruno, G, et al. 2020. β3-adrenoreceptor blockade reduces tumor growth and increases neuronal differentiation in neuroblastoma via SK2/S1P2 modulation. Oncogene, 368-384.

Western Blot: Mouse
PMID	Dilution	Publication
33942347	not listed	Pei, G, et al. 2021. Cellular stress promotes NOD1/2-dependent inflammation via the endogenous metabolite sphingosine-1-phosphate. The EMBO journal, e106272.

Western Blot: Rat
PMID	Dilution	Publication
36496062	1:1000	Mazzoli, A, et al. 2023. Skeletal Muscle Insulin Resistance and Adipose Tissue Hypertrophy Persist Beyond the Reshaping of Gut Microbiota in Young Rats Fed a Fructose-rich Diet. The Journal of Nutritional Biochemistry, 109247.

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Anti-Sphingosine Kinase 2 (N-terminal region) Antibody