Anti-Sphingosine Kinase 1 (Ser-225), Phosphospecific Antibody

Name: Anti-Sphingosine Kinase 1 (Ser-225), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: SP1641
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

34 Citations

Product Type

Primary Antibody

Species Reactivity

Human

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

SPHK1

Molecular Weight

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Western blot image of recombinant his-tagged human SK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-SK1 (Central Region) (SP1621; lanes 1-3) and anti-SK1 (Ser-225) (SP1641; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-SK1 (Ser-225) peptide (SX1645; lanes 2 & 5), or unphosphorylated SK1 (Ser-225) peptide (SX1625; lanes 3 & 6).

Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK1 (Central region) SP1621 (lanes 1 & 2) or anti-SK1 (Ser-225) SP1641 (lanes 3 & 4).

The protein expression levels of DGKα, SphK1 (cat. SP5421, 1:1000) and p-SphK1(Ser225) (cat. SP1641) in AML cells after ritanserin treatment for 48 h. Image from publication CC-BY-4.0. PMID: 37392305

Western blot image of recombinant his-tagged human SK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-SK1 (Central Region) (SP1621; lanes 1-3) and anti-SK1 (Ser-225) (SP1641; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-SK1 (Ser-225) peptide (SX1645; lanes 2 & 5), or unphosphorylated SK1 (Ser-225) peptide (SX1625; lanes 3 & 6).

Volume: 100 µL

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Product Details

Target

Sphingosine Kinase 1 (Ser-225)

Target Description

Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

SPHK1

Molecular Weight

Antigen

Phospho-SK1 (Ser-225) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 225 in human SK1. This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and is not homologous to sequences in SK2. The conserved site in rat and mouse SK1 is at serine 224.

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to non-specific phosphoserine and unphosphorylated SK1 (Ser-225) peptide before affinity purification using phospho-SK1 (Ser-225) peptide. The purified antibody detects a 47 kDa* full-length recombinant human SK1 protein phosphorylated with ERK2.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-225

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SK1, SPHK1, SPK1

UniProt Details

UniProt (Human): Q9NYA1
UniProt (Immunogen Species): Q9NYA1

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse
Immunohistochemistry: Human | Mouse | Rat
Western Blot: Human | Mouse
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
34873055	not listed	Molinar-Inglis, O, et al. 2021. aPC/PAR1 confers endothelial anti-apoptotic activity via a discrete, β-arrestin-2-mediated SphK1-S1PR1-Akt signaling axis. Proceedings of the National Academy of Sciences of the United States of America, e2106623118.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
32771459	not listed	Ranasinghe, ADCU, et al. 2020. Mechanistic regulation of SPHK1 expression and translocation by EMAP II in pulmonary smooth muscle cells. Biochimica et biophysica acta. Molecular and cell biology of lipids, 158789.

Immunohistochemistry: Human
PMID	Dilution	Publication
37648331	1:100	Nagaro, H, et al. 2023. Clinical Significance of Phosphorylated Sphingosine Kinase 1 Expression in Pancreatic Ductal Adenocarcinoma. Anticancer Research, 3969-3977.
30527463	1:100	Nemoto, M, et al. 2019. Phospho-Sphingosine Kinase 1 Expression in Lymphatic Spread of Esophageal Squamous Cell Carcinoma. The journal of surgical research, 123-131.
30278948	1:100	Yuza, K, et al. 2018. Upregulation of phosphorylated sphingosine kinase 1 expression in colitis-associated cancer. The journal of surgical research, 323-330.
29370930	1:100	Hanyu, T, et al. 2018. Expression of phosphorylated sphingosine kinase 1 is associated with diffuse type and lymphatic invasion in human gastric cancer. Surgery, 1301-1306.
27864331	1:200	Fu, P, et al. 2016. Role of Sphingosine Kinase 1 and S1P Transporter Spns2 in HGF-mediated Lamellipodia Formation in Lung Endothelium. The journal of biological chemistry, 27187-27203.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
29523764	not listed	Yamada, A, et al. 2018. ABCC1-Exported Sphingosine-1-phosphate, Produced by Sphingosine Kinase 1, Shortens Survival of Mice and Patients with Breast Cancer. Molecular cancer research : MCR, 1059-1070.

Immunohistochemistry: Rat
PMID	Dilution	Publication
31371480	not listed	Alganga, H, et al. 2019. Short Periods of Hypoxia Upregulate Sphingosine Kinase 1 and Increase Vasodilation of Arteries to Sphingosine 1-Phosphate (S1P) via S1P3. The journal of pharmacology and experimental therapeutics, 63-74.

Western Blot: Human
PMID	Dilution	Publication
37582160	1:1000	Bergkamp, ND, et al. 2023. A virally encoded GPCR drives glioblastoma through feed-forward activation of the SK1-S1P1 signaling axis. Science Signaling, eade6737.
36358599	not listed	Hengst, JA, et al. 2022. Regulatory Role of Sphingosine-1-Phosphate and C16:0 Ceramide, in Immunogenic Cell Death of Colon Cancer Cells Induced by Bak/Bax-Activation. Cancers, 5182.
35159039	not listed	Petrusca, DN, et al. 2022. GFI1-Dependent Repression of SGPP1 Increases Multiple Myeloma Cell Survival. Cancers, 772.
33931106	not listed	Dai, L, et al. 2021. Activation of SphK1 by adipocytes mediates epithelial ovarian cancer cell proliferation. Journal of ovarian research, 62.
33525436	not listed	Bernacchioni, C, et al. 2021. Role of Sphingosine 1-Phosphate Signalling Axis in Muscle Atrophy Induced by TNFα in C2C12 Myotubes. International journal of molecular sciences, 1280.
31413745	not listed	Wu, X, et al. 2019. SphK1 functions downstream of IGF-1 to modulate IGF-1-induced EMT, migration and paclitaxel resistance of A549 cells: A preliminary in vitro study. Journal of cancer, 4264-4269.
31363040	1:1000	Kao, LP, et al. 2019. Chemotherapy selection pressure alters sphingolipid composition and mitochondrial bioenergetics in resistant HL-60 cells. Journal of lipid research, 1590-1602.
30118478	not listed	Arish, M, et al. 2018. Sphingosine-1-phosphate signaling in Leishmania donovani infection in macrophages. PLoS neglected tropical diseases, e0006647.
29427528	1:500	Cattaneo, MG, et al. 2018. Cross-talk between sphingosine-1-phosphate and EGFR signaling pathways enhances human glioblastoma cell invasiveness. FEBS letters, 949-961.
28240350	1:1000	Lee, HM, et al. 2017. Oncogenic S1P signalling in EBV-associated nasopharyngeal carcinoma activates AKT and promotes cell migration through S1P receptor 3. The journal of pathology, 62-72.
27956387	not listed	Powell, J, et al. 2017. Targeting sphingosine kinase 1 induces MCL1-dependent cell death in acute myeloid leukemia. Blood, 771-782.
27864331	not listed	Fu, P, et al. 2016. Role of Sphingosine Kinase 1 and S1P Transporter Spns2 in HGF-mediated Lamellipodia Formation in Lung Endothelium. The journal of biological chemistry, 27187-27203.
24137500	not listed	Seo, YJ, et al. 2013. Sphingosine kinase 1 serves as a pro-viral factor by regulating viral RNA synthesis and nuclear export of viral ribonucleoprotein complex upon influenza virus infection. PLoS One, e75005.
20634980	not listed	Benakanakere, MR, et al. 2010. Sphingosine kinase-1 is required for toll mediated beta-defensin 2 induction in human oral keratinocytes. PLoS One, e11512.
18426913	not listed	Miller, AV, et al. 2008. Sphingosine kinases and sphingosine-1-phosphate are critical for transforming growth factor beta-induced extracellular signal-regulated kinase 1 and 2 activation and promotion of migration and invasion of esophageal cancer cells. Molecular and cellular biology, 4142-4151.

Western Blot: Mouse
PMID	Dilution	Publication
37392305	not listed	Tan ,J, et al. 2023. Ritanserin suppresses acute myeloid leukemia by inhibiting DGKα to downregulate phospholipase D and the Jak-Stat/MAPK pathway. Discover. Oncology, 118.
35203362	not listed	Bernacchioni, C, et al. 2022. S1P Signalling Axis Is Necessary for Adiponectin-Directed Regulation of Electrophysiological Properties and Oxidative Metabolism in C2C12 Myotubes. Cells, 713.
32771459	not listed	Ranasinghe, ADCU, et al. 2020. Mechanistic regulation of SPHK1 expression and translocation by EMAP II in pulmonary smooth muscle cells. Biochimica et biophysica acta. Molecular and cell biology of lipids, 158789.
31971297	not listed	Mohammed, S, et al. 2020. Regulatory role of SphK1 in TLR7/9-dependent type I interferon response and autoimmunity. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 4329-4347.
30569280	not listed	Grassi, S, et al. 2019. Human Remyelination Promoting Antibody Stimulates Astrocytes Proliferation Through Modulation of the Sphingolipid Rheostat in Primary Rat Mixed Glial Cultures. Neurochemical research, 1460-1474.
30279138	1:1000	Pierucci, F, et al. 2018. Involvement of released sphingosine 1-phosphate/sphingosine 1-phosphate receptor axis in skeletal muscle atrophy. Biochimica et biophysica acta. Molecular basis of disease, 3598-3614.
28890935	1:1000	Hengst, J, et al. 2017. SKI-178: A Multitargeted Inhibitor of Sphingosine Kinase and Microtubule Dynamics Demonstrating Therapeutic Efficacy in Acute Myeloid Leukemia Models. Cancer translational medicine, 109-121.
27485122	1:500	Salta, E, et al. 2016. miR-132 loss de-represses ITPKB and aggravates amyloid and TAU pathology in Alzheimer's brain. EMBO molecular medicine, 1005-1018.
24464131	not listed	Harikumar, KB, et al. 2014. K63-linked polyubiquitination of transcription factor IRF1 is essential for IL-1-induced production of chemokines CXCL10 and CCL5. Nature immunology, 231-238.

Additional Publications: Unspecified
PMID	Publication
37225163	Medina-Moctezuma, ZB, et al. 2023. Sphingosine-1-phosphate Regulation of Luteinising Hormone-induced Steroidogenesis and Proliferation of Bovine Theca Cells in Vitro. Reproduction, Fertility, and Development, 518-526.

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Anti-Sphingosine Kinase 1 (Ser-225), Phosphospecific Antibody