Our Anti-SOX2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse SOX2 and is antigen affinity purified from pooled serum. It is an excellent marker of neural stem cells.
Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~35 kDa SOX2 protein.
SOX2 (SRY (sex-determining region Y)-box 2) is one of 20 members of the Sox family proteins that play essential roles in cell differentiation, development, and organogenesis. SOX2, a group B Sox family protein, is one of the master transcriptional factors, along with Oct3/4 (Nichols, J., et al, 1998) and PAK6 (Kamachi, Y. et al, 2001) that are important in maintaining the pluripotency of embryonic stem cells, thus making SOX2 an excellent marker of neural stem cells. SOX2 has also been identified as a lineage-survival oncogene in lung and esophageal squamous cell carcinomas due to peak amplification on chromosome 3q26.33 which contains the SOX2 gene transcription factor (Bass, A.J., et al, 2009). Additionally, Sox2 has been reported to be highly deregulated in glioblastomas (Gangemi, R. M. R, et al, 2009) and breast cancer (Rodriguez-Pinilla, S.M., et al, 2007).
Antigen Affinity Purified from Pooled Serum
Recombinant protein from the C-terminal region of human SOX2.
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from rabbit serum by affinity purification using a column to which the recombinant protein imunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Specific for endogenous levels of the ~35 kDa SOX2 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.