Anti-SOX2 Antibody

Name: Anti-SOX2 Antibody
Brand: PhosphoSolutions
SKU: 1907-SOX2
Price: 415.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Trial Size

Pooled Serum

Our Anti-SOX2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse SOX2 and is antigen affinity purified from pooled serum. It is an excellent marker of neural stem cells.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse

Expected Reactivity

Human

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SOX2

Molecular Weight

35 kDa

Go to Full Product Details

Immunofluoresence of FLAG-tagged SOX2 transfected COS cells showing SOX2 staining in red (cat. 1907-SOX2, 1:20) and FLAG staining in green, causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.

Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~35 kDa SOX2 protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-SOX2 Antibody

Product Details

Target

SOX2

Target Description

SOX2 (SRY (sex-determining region Y)-box 2) is one of 20 members of the Sox family proteins that play essential roles in cell differentiation, development, and organogenesis. SOX2, a group B Sox family protein, is one of the master transcriptional factors, along with Oct3/4 (Nichols, J., et al, 1998) and PAK6 (Kamachi, Y. et al, 2001) that are important in maintaining the pluripotency of embryonic stem cells, thus making SOX2 an excellent marker of neural stem cells. SOX2 has also been identified as a lineage-survival oncogene in lung and esophageal squamous cell carcinomas due to peak amplification on chromosome 3q26.33 which contains the SOX2 gene transcription factor (Bass, A.J., et al, 2009). Additionally, Sox2 has been reported to be highly deregulated in glioblastomas (Gangemi, R. M. R, et al, 2009) and breast cancer (Rodriguez-Pinilla, S.M., et al, 2007).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

SOX2

Molecular Weight

35 kDa

Antigen

Recombinant protein from the C-terminal region of human SOX2.

Species Reactivity

Human, Mouse

Expected Reactivity

Human

Antibody Registry ID

AB_2868513

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification using a column to which the recombinant protein imunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
ICC: 1:20-1:200

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~35 kDa SOX2 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SRY box 2, sex-determining region Y box 2

UniProt Details

UniProt (Human): P48431
UniProt (Immunogen Species): P48431

NCBI Gene ID

6657

Product Specific Protocols

Western Blot Protocol: Download

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Anti-SOX2 Antibody

Our Anti-SOX2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse SOX2 and is antigen affinity purified from pooled serum. It is an excellent marker of neural stem cells.