Anti-SHP1 (Ser-591), Phosphospecific Antibody
Our SHP1 (Ser-591) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat SHP1 (Ser-591) and is antigen affinity purified. It is great for use in WB.
Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Ser-591) antibody (lanes 3-6). The SHP1 (Ser-591) antibody was used in the presence of phospho-SHP1 (Ser-591) peptide (lane 5) or a non-specific phospho-serine peptide (lane 6).
Product Specific References for Applications and Species
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 18617527 | not listed | Simoneau, M, et al. 2008. Activation of Cdk2 stimulates proteasome-dependent truncation of tyrosine phosphatase SHP-1 in human proliferating intestinal epithelial cells. The Journal of Biological Chemistry, 25544-25556. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 35258455 | 1:500 | Ben-Shmuel, A, et al. 2022. Inhibition of SHP-1 activity by PKC-θ regulates NK cell activation threshold and cytotoxicity. Elife, e73282. |
| 33846641 | 1:2000 | Weng, JH, et al. 2021. Colchicine acts selectively in the liver to induce hepatokines that inhibit myeloid cell activation. Nature Metabolism, 513-522. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 24947019 | not listed | Mani, R, et al. 2015. Tumor antigen ROR1 targeted drug delivery mediated selective leukemic but not normal B-cell cytotoxicity in chronic lymphocytic leukemia. Leukemia, 346-355. |
| 23720348 | not listed | Zhou, X, et al. 2013. High NaCl-induced inhibition of PTG contributes to activation of NFAT5 through attenuation of the negative effect of SHP-1. American Journal of Physiology. Renal Physiology, F362-369. |
| 21527515 | not listed | Salas, A, et al. 2011. Sphingosine kinase-1 and sphingosine 1-phosphate receptor 2 mediate Bcr-Abl1 stability and drug resistance by modulation of protein phosphatase 2A. Blood, 5941-5952. |
| 18617527 | not listed | Simoneau, M, et al. 2008. Activation of Cdk2 stimulates proteasome-dependent truncation of tyrosine phosphatase SHP-1 in human proliferating intestinal epithelial cells. The Journal of Biological Chemistry, 25544-25556. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 35258455 | 1:1000 | Ben-Shmuel, A, et al. 2022. Inhibition of SHP-1 activity by PKC-θ regulates NK cell activation threshold and cytotoxicity. Elife, e73282. |
| 33846641 | 1:2000 | Weng, JH, et al. 2021. Colchicine acts selectively in the liver to induce hepatokines that inhibit myeloid cell activation. Nature Metabolism, 513-522. |
| 31033226 | not listed | Wang, H, et al. 2019. Sickle cell disease up-regulates vasopressin, aquaporin 2, urea transporter A1, Na-K-Cl cotransporter 2, and epithelial Na channels in the mouse kidney medulla despite compromising urinary concentration ability. Physiological reports, e14066. |
| 30373850 | not listed | Ormonde, J, et al. 2018. TAOK3 Regulates Canonical TCR Signaling by Preventing Early SHP-1-Mediated Inactivation of LCK. Journal of immunology (Baltimore, Md. : 1950), 3431-3442. |
| 27271569 | not listed | Moogk, D, et al. 2016. Constitutive Lck Activity Drives Sensitivity Differences between CD8+ Memory T Cell Subsets. Journal of immunology (Baltimore, Md. : 1950), 644-654. |
| 25391900 | not listed | Wang, H, et al. 2015. PKC-α contributes to high NaCl-induced activation of NFAT5 (TonEBP/OREBP) through MAPK ERK1/2. American Journal of Physiology. Renal Physiology, F140-148. |
| 24990897 | not listed | Zhou, X, et al. 2014. Leishmania infantum-chagasi activates SHP-1 and reduces NFAT5/TonEBP activity in the mouse kidney inner medulla. American Journal of Physiology. Renal Physiology, F516-524. |
| 20351292 | not listed | Zhou, X, et al. 2010. Contribution of SHP-1 protein tyrosine phosphatase to osmotic regulation of the transcription factor TonEBP/OREBP. Proceedings of the National Academy of Sciences of the United States of America, 7072-7077. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 24140598 | not listed | Tibaldi, E, et al. 2014. The tyrosine phosphatase SHP-1 inhibits proliferation of activated hepatic stellate cells by impairing PDGF receptor signaling. Biochimica et Biophysica acta, 288-298. |