Western blot analysis of ShcA expression in A431 cell lysate (lanes 1, 2, 3, & 4). The blots were probed with rabbit polyclonal anti-ShcA (SP1331) at 1:1000 (lane 1) or 1:4000 (lane 2) and mouse monoclonal ShcA (C-terminal region) at 1:1000 (lane 3) or 1:4000 (lane 4).
Anti-ShcA Antibody
SKU: SP1331
Bulk Order Anti-ShcA Antibody
Product Details
ShcA
The adapter protein Shc was initially identified as an SH2 containing proto-oncogene involved in growth factor signaling. Since then, a number of studies in multiple systems have implicated a role for Shc in many different types of signal transduction including growth factor, antigen, cytokine, G-protein, hormone, and integrin receptor signaling. In addition to the ubiquitous ShcA, there are two other shc gene products, ShcB and ShcC, which are predominantly expressed in neuronal cells. ShcA knockout mice are embryonic lethal and have clearly suggested an important role for ShcA in vivo. An important role for Shc in the activation of MAPK pathway has been established. Thus, Shc adapter proteins are critical components of signal transduction pathways involved in many different cellular processes.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
SHC1
45/52/66
A recombinant protein that included amino acid residues within the C-terminal region of human ShcA. This sequence has high homology with similar regions in rat and mouse ShcA.
Human, Mouse, Rabbit, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:50
Unconjugated
The antibody detects 46/52/66 kDa* bands corresponding to the molecular weight of ShcA variants on SDS-PAGE immunoblots of A431 cells. It also detects ShcA in human Jurkat, HeLa, and SKN-SH cells, as well as rat brain tissue and rabbit spleen fibroblasts.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
