Our Semaphorin-6A (cytoplasmic region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Semaphorin-6A (cytoplasmic region) and is antigen affinity purified. It is great for use in WB, ICC, IP.
Western blot of Sema-6A in human colorectal cancer (CRC) cells treated with control (shC) or Sema-6A (shSema6A) shRNAs. Sema-6A was immunoprecipitated from each of the CRC lysates using Sema-6A (a.a. 772-787) or Sema-6A (C-terminus) antibody, then the blotted immunoprecipitations were probed with Sema-6A antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, Univ. of Torino, Italy).
The Semaphorin family of axon guidance molecules includes secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 6A (Sema-6A) is a single-pass type I membrane protein that contains a plexin-semaphorin-integrin (PSI) domain and a Sema domain. Sema-6A is active as a homodimer or oligomer. The Sema-6A homodimer interacts with a Plexin A2 homodimer. Sema-6a promotes reorganization of the actin cytoskeleton to regulate normal granule cell migration in the developing cerebellum and to control axon guidance in the developing central nervous system. In addition, Sema-6a has been implicated in oligodendrocyte myelination and may alter VEGF signaling during angiogenesis.
Antigen Affinity Purified
Polyclonal
IgG
ICC, IP, WB
Rabbit
SEMA6A
120
Semaphorin 6A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the cytoplasmic region of human Sema-6A. The sequence used has high homology to similar regions in rat and mouse Sema-6A, and has low homology to other semaphorin family members.
Chicken, Frog, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:250
IP: 1:100
Unconjugated
The antibody detects a 120kDa* protein corresponding to the apparent molecular mass of Sema-6A on SDS-PAGE immunoblots of Sema-6A transfected COS7 cells, human colorectal cancer cells, and rat PC12 cells. In addition, shRNA knockdown of Sema-6A expression significantly reduces detection of Sema-6A using anti-Sema-6A (a.a. 772-787) antibody.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
from PhosphoSolutions