Our Semaphorin-3F (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Semaphorin-3F (C-terminal region) and is antigen affinity purified. It is great for use in WB.
Western blot analysis of Sema-3F expression in adult mouse brain (lane 1), kidney (lane 2), and liver (lane 3). The blot was probed with anti-Sema-3F (SP3921) at 1:1000.
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3F (Sema-3F) is a class III secreted semaphorin that binds with high affinity to Neuropilin-2, and low affinity to Neuropilin-1. During peripheral and central nervous system development, Sema-3F has critical roles in axon guidance and dendrite outgrowth. Sema-3F has also been shown to inhibit angiogenesis by manipulating VEGFR function and decreasing blood vessel density. In cancers, Sema-3F induces a poorly vascularized, encapsulated, non-metastatic phenotype through chemorepulsion of endothelial cells in melanoma, while Sema-3F disrupts intercellular contacts of MCF7 breast cancer cells through delocalization of E-cadherin and β-catenin. Thus, Sema-3F may have important roles in axon guidance, angiogenesis, and tumor progression
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
SEMA3F
95
Semaphorin 3F synthetic peptide (coupled to carrier protein) corresponds to amino acids from the C-terminal region of human Sema-3F. This sequence is highly conserved in rat and mouse Sema-3F, and has low homology to other semaphorin family members.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
Unconjugated
The antibody detects a 95 kDa* protein corresponding to the apparent molecular mass of Sema-3F on SDS-PAGE immunoblots of adult mouse brain and liver.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
