Anti-Semaphorin-3A (Central region) Antibody

Name: Anti-Semaphorin-3A (Central region) Antibody
Brand: PhosphoSolutions
SKU: SP1221
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

SEMA3A

Molecular Weight

Go to Full Product Details

Formalin fixed, citric acid treated parafin sections of adult Rat cerebellum. Sections were probed with anti-Sema3A (SP1221) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Sema3A.

Volume: 100 µL

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Product Details

Target

Semaphorin-3A (Central region)

Target Description

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

SEMA3A

Molecular Weight

Antigen

Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the central region of human Sema3A. The sequence used is highly conserved in rat and mouse Sema3A, and has low homology to other semaphorin family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 6:00
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A and neonatal rat brain lysates.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Sema3A, Semaphorin III

UniProt Details

UniProt (Human): Q14563
UniProt (Immunogen Species): Q14563

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Anti-Semaphorin-3A (Central region) Antibody