Western blot image of human A431 cells and (lane 1) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lane 2). The blots were probed with rabbit polyclonal anti-Semaphorin 3A (SP1241) at a dilution of 1:1000.
Anti-Semaphorin-3A (C-terminal) Antibody
Bulk Order Anti-Semaphorin-3A (C-terminal) Antibody
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
Antigen Affinity Purified
Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the C-terminal region of human Sema3A. The sequence used has two amino acid differences from rat and mouse Sema3A, and has low homology to other semaphorin family members.
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A/Fc chimera and human A431 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.