Anti-RCAN1/Dscr1 (C-terminus) Antibody

Name: Anti-RCAN1/Dscr1 (C-terminus) Antibody
Brand: PhosphoSolutions
SKU: RP3941
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

2 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

RCAN1

Molecular Weight

31/38

Go to Full Product Details

Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (RP3941) and anti-RCAN1 (a.a. 132-140) (RP3961) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide RX3945 and RX3965, respectively. The antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot analysis of RCAN1 expression in human Jurkat (lane 1), rat PC12 (lane 2), human A431 (lane 3), and adult mouse muscle (lane 4). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000.

Volume: 100 µL

Variant

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$309

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Product Details

Target

RCAN1/Dscr1 (C-terminus)

Target Description

An important element of calcium signaling pathways involves calmodulin activation of calcineurin (phosphatase PP2B), leading to dephosphorylation of transcription factors such as NFAT and MEF2. A wide variety of proteins other than calmodulin have also been implicated in regulating calcineurin activity. Regulators of Calcineurin (RCANs) include RCAN1, RCAN2, and RCAN3. RCAN1 has previously been referred to as Down's syndrome candidate region-1 (Dscr1), MCIP, calcipressin, and Adapt78. This RCAN is expressed as several different variants with RCAN1L (38 kDa) and RCAN1S (31 kDa) being most prevalent. RCAN1 is increased in Down's syndrome tissues and in a mouse model of Down's syndrome. Increased expression of RCAN1 leads to significant suppression of tumor growth in mice as result of deficits in calcineurin-induced tumor angiogenesis. RCAN1 can recruit TAB1, TAK1, and calcineurin into a macromolecular signaling complex, and TAK1 can phosphorylate Ser-94 and Ser-136 in RCAN1S. This phosphorylation converts RCAN1 from an inhibitor to a facilitator of calcineurin-NFAT signaling.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

RCAN1

Molecular Weight

31/38

Antigen

RCAN1 (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminus of human RCAN1. This sequence is highly conserved in rat and mouse RCAN1, and this sequence is found in all four isoforms of RCAN1, but is not found in RCAN2 or RCAN3.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects several isoforms of RCAN1 on SDS-PAGE immunoblots of adult mouse muscle and brain, rat PC12 cells, and human A431 and Jurkat cells. The predominate isoforms detected were the 38 kDa RCAN1L and 31 kDa RCAN1S.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Dscr1, MCIP, RCAN1, calcipressin, Adapt78

UniProt Details

UniProt (Human): P53805
UniProt (Immunogen Species): P53805

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Human

Immunocytochemistry: Mouse
PMID	Dilution	Publication
36450444	1:100	Choi, C, et al. 2022. DSCR1 deficiency ameliorates the Aβ pathology of Alzheimer's disease by enhancing microglial activity. Life Science Alliance, e202201556.

Western Blot: Human
PMID	Dilution	Publication
28553204	not listed	Han, K, et al. 2017. Leucine-Rich Repeat Kinase 2 (LRRK2) Stimulates IL-1β-Mediated Inflammatory Signaling through Phosphorylation of RCAN1. Frontiers in cellular neuroscience, 125.

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Anti-RCAN1/Dscr1 (C-terminus) Antibody