Western blot analysis of RCAN1 expression in human Jurkat (lane 1), rat PC12 (lane 2), human A431 (lane 3), and adult mouse muscle (lane 4). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000.
Anti-RCAN1/Dscr1 (C-terminus) Antibody
Bulk Order Anti-RCAN1/Dscr1 (C-terminus) Antibody
An important element of calcium signaling pathways involves calmodulin activation of calcineurin (phosphatase PP2B), leading to dephosphorylation of transcription factors such as NFAT and MEF2. A wide variety of proteins other than calmodulin have also been implicated in regulating calcineurin activity. Regulators of Calcineurin (RCANs) include RCAN1, RCAN2, and RCAN3. RCAN1 has previously been referred to as Down's syndrome candidate region-1 (Dscr1), MCIP, calcipressin, and Adapt78. This RCAN is expressed as several different variants with RCAN1L (38 kDa) and RCAN1S (31 kDa) being most prevalent. RCAN1 is increased in Down's syndrome tissues and in a mouse model of Down's syndrome. Increased expression of RCAN1 leads to significant suppression of tumor growth in mice as result of deficits in calcineurin-induced tumor angiogenesis. RCAN1 can recruit TAB1, TAK1, and calcineurin into a macromolecular signaling complex, and TAK1 can phosphorylate Ser-94 and Ser-136 in RCAN1S. This phosphorylation converts RCAN1 from an inhibitor to a facilitator of calcineurin-NFAT signaling.
Antigen Affinity Purified
RCAN1 (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminus of human RCAN1. This sequence is highly conserved in rat and mouse RCAN1, and this sequence is found in all four isoforms of RCAN1, but is not found in RCAN2 or RCAN3.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
The antibody detects several isoforms of RCAN1 on SDS-PAGE immunoblots of adult mouse muscle and brain, rat PC12 cells, and human A431 and Jurkat cells. The predominate isoforms detected were the 38 kDa RCAN1L and 31 kDa RCAN1S.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.