Name: Anti-Rabphilin 3A (Ser234) Antibody
Brand: PhosphoSolutions
SKU: p1553-234
Price: 415.0 USD
Availability: InStock

Our Anti-Rabphilin 3A (Ser234) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Rabphilin 3A (Ser234) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Mouse

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

RPH3A

Molecular Weight

82 kDa

Go to Full Product Details

Immunostaining of 14 DIV rat cortical neurons labeling rabphilin when phosphorylated at Ser234 (cat. p1553-234, red, 1:400) and PSD95 (green). The rat cortical neurons were fixed with methanol at -20C for 10 minutes. Photo courtesy of Gang Liu.

Western blot of rat brain lysate showing specific immunolabeling of the ~82 kDa rabphilin 3A phosphorylated at Ser234.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Rabphilin 3A (Ser234) Antibody

Product Details

Target

Rabphilin 3A (Ser234)

Target Description

Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994; Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser-234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995; Foletti and Scheller, 2001).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

RPH3A

Molecular Weight

82 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of rat rabphilin 3A, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Mouse

Antibody Registry ID

AB_2492222

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:400

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~82 kDa rabphilin protein phosphorylated at Ser234. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser234

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Exophilin 1 antibody, Exophilin-1 antibody, Exophilin1 antibody, KIAA0985 antibody, Rabphilin 3A homolog antibody, Rabphilin-3A antibody, Rabphilin3A antibody, RP3A antibody, RP3A_HUMAN antibody, Rph 3a antibody, Rph3a antibody

UniProt Details

UniProt (Immunogen Species): P47709

NCBI Gene ID

171039

Product Specific References for Applications and Species

Western Blot: Rat

Western Blot: Rat
PMID	Dilution	Publication
28823933	not listed	Stanic, J., et al. 2017. Rabphilin 3A: A novel target for the treatment of levodopa-induced dyskinesias. Neurobiology of Disease, 108, pp.54-64.
18519737	not listed	Leal-Ortiz, S., et al. 2008. Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. The Journal of Cell Biology, 181(5), pp.831-846.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Rabphilin 3A (Ser234) Antibody

Our Anti-Rabphilin 3A (Ser234) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Rabphilin 3A (Ser234) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.