Name: Anti-Rabphilin 3A Antibody
Brand: PhosphoSolutions
SKU: 1825-RBP
Price: 415.0 USD
Availability: InStock

Our Anti-Rabphilin 3A rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Rabphilin 3A and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate, Mouse

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

RPH3A

Molecular Weight

82 kDa

Go to Full Product Details

Western blot of rat cortical lysate showing specific immunolabeling of the ~82 kDa rabphilin 3A.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Rabphilin 3A Antibody

Product Details

Target

Rabphilin 3A

Target Description

Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser-234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

RPH3A

Molecular Weight

82 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat rabphilin 3A conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate, Mouse

Antibody Registry ID

AB_2492221

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~82 kDa rabphilin 3A protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Exophilin 1 antibody, Exophilin-1 antibody, Exophilin1 antibody, KIAA0985 antibody, Rabphilin 3A homolog antibody, Rabphilin-3A antibody, Rabphilin3A antibody, RP3A antibody, RP3A_HUMAN antibody, Rph 3a antibody, Rph3a antibody

UniProt Details

UniProt (Human): Q9Y2J0
UniProt (Immunogen Species): P47709

NCBI Gene ID

171039

Product Specific Protocols

Western Blot Protocol: Download

General References

Foletti, D.L. and Scheller, R.H., 2001. Developmental regulation and specific brain distribution of phosphorabphilin. Journal of Neuroscience, 21(15), pp.5461-5472. PMID: 11466417
Fykse, E.M., Li, C. and Sudhof, T.C., 1995. Phosphorylation of rabphilin-3A by Ca2+/calmodulin-and cAMP-dependent protein kinases in vitro. Journal of Neuroscience, 15(3), pp.2385-2395. PMID: 7891174
Geppert, M., Bolshakov, V.Y., Siegelbaum, S.A., Takei, K., De Camilli, P., Hammer, R.E. and Südhof, T.C., 1994. The role of Rab3A in neurotransmitter release. Nature, 369(6480), p.493-7. PMID: 7911226
Li, C., Takei, K., Geppert, M., Daniell, L., Stenius, K., Chapman, E.R., Jahn, R., De Camilli, P. and Südhof, T.C., 1994. Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C. Neuron, 13(4), pp.885-898. PMID: 7946335
Smith, R., Petersén, Å., Bates, G.P., Brundin, P. and Li, J.Y., 2005. Depletion of rabphilin 3A in a transgenic mouse model (R6/1) of Huntington's disease, a possible culprit in synaptic dysfunction. Neurobiology of Disease, 20(3), pp.673-684. PMID: 15967669

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Anti-Rabphilin 3A Antibody

Our Anti-Rabphilin 3A rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Rabphilin 3A and is antigen affinity purified from pooled serum. It is great for use in WB.