Name: Anti-Rab 3 Antibody
Brand: PhosphoSolutions
SKU: 1805-Rab3
Price: 415.0 USD
Availability: InStock

Our Anti-Rab 3 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Rab 3 and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Human, Mouse, Non-Human Primate, Xenopus

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

RAB3A

Molecular Weight

27 kDa

Go to Full Product Details

Western blot of rat cortical lysate showing specific labeling of the ~27 kDa Rab3 protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Rab 3 Antibody

Product Details

Target

Rab 3

Target Description

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D (Schluter et al., 2004). These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters (Coppola et al., 2002). Specifically, Rab3’s are thought to play a key role in presynaptic vesicle trafficking and priming (Schluter et al., 2006).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

RAB3A

Molecular Weight

27 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat Rab 3, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Human, Mouse, Non-Human Primate, Xenopus

Antibody Registry ID

AB_2492220

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column made with the N-terminal peptide used as antigen.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~27 kDa Rab3 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Rab 3A antibody, RAB 3A member RAS oncogene family antibody, Rab3a antibody, RAB3A member RAS oncogene family antibody, RAB3A_HUMAN antibody, RAS associated protein RAB 3A antibody, RAS associated protein RAB3A antibody, Ras related protein Rab 3A antibody, Ras related protein Rab3A antibody, Ras-related protein Rab-3A antibody

UniProt Details

UniProt (Human): P20336
UniProt (Immunogen Species): P63012

NCBI Gene ID

25531

Product Specific Protocols

Western Blot Protocol: Download

General References

Schlüter, O.M., Schmitz, F., Jahn, R., Rosenmund, C. and Südhof, T.C., 2004. A complete genetic analysis of neuronal Rab3 function. Journal of Neuroscience, 24(29), pp.6629-6637. PMID: 15269275
Schlüter, O.M., Basu, J., Südhof, T.C. and Rosenmund, C., 2006. Rab3 superprimes synaptic vesicles for release: implications for short-term synaptic plasticity. Journal of Neuroscience, 26(4), pp.1239-1246. PMID: 16436611
Coppola, T., Perret-Menoud, V., Gattesco, S., Magnin, S., Pombo, I., Blank, U. and Regazzi, R., 2002. The death domain of Rab3 guanine nucleotide exchange protein in GDP/GTP exchange activity in living cells. Biochemical Journal, 362(2), pp.273-279. PMID: 11853534

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Anti-Rab 3 Antibody

Our Anti-Rab 3 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Rab 3 and is antigen affinity purified from pooled serum. It is great for use in WB.