Anti-Profilin (Tyr-129), Phosphospecific Antibody

Name: Anti-Profilin (Tyr-129), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: PP4751
Price: 329.0 USD
Availability: InStock

2 Citations

Our Profilin (Tyr-129) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Profilin (Tyr-129) and is antigen affinity purified. It is great for use in WB.

Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: PP4751

Product Details

Target

Profilin (Tyr-129)

Target Description

Profilins are small actin-binding proteins that have functions in cell motility, cytokinesis, gene transcription, endocytosis and neuronal plasticity. Four profilin isoforms have been identified in mammals. Profilin-1 (PFN1) and profilin-2a (PFN2a) isoforms are highly conserved in structure, but PFN1 is ubiquitously expressed while PFN2a is preferentially enriched in brain. In addition, there are two testis-specific profilins, PFN3 and PFN4, that significantly differ in primary sequence and function compared to PFN1 and PFN2a. Profilin is phosphorylated at both tyrosine and serine residues in vivo. Tyr-129 is phosphorylated in response to VEGF-A stimulation, and this promotes profilin actin binding and polymerization. Tyr-129 phosphorylation may be important for angiogenesis induced by injuries. Ser-138 is phosphorylated by ROCK and dephosphorylated by PP1. This serine phosphorylation inhibits G-actin binding, as well as decreases profilin's aggregation suppressor activity by inhibiting binding to huntingtin. Thus, Tyr-129 phosphorylation may activate while Ser-138 phosphorylation may inhibit profilin activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

PFN1

Molecular Weight

Antigen

Phospho-Profilin (Tyr-129) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Tyr-129 in human Profilin-1. This sequence is well conserved in rat and mouse Profilin-1. The tyrosine site has some homology to the conserved site (Tyr-129) in Profilin-2a and Profilin-2b, but the site is not conserved in Profiin-3 and Profilin-4.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to unphosphorylated Profilin (Tyr-129) then affinity purified using Profilin (Tyr-129) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human umbilical vein endothelial cells (HUVEC) and jurkat cells treated with pervanadate. This reactivity is removed by alkaline phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-129

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Epididymis Li184a Profilin PFN1 PFN2

UniProt Details

UniProt (Human): P07737
UniProt (Immunogen Species): P07737

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
29163025	1:300	Dombert, B, et al. 2017. BDNF/trkB Induction of Calcium Transients through Cav2.2 Calcium Channels in Motoneurons Corresponds to F-actin Assembly and Growth Cone Formation on β2-Chain Laminin (221). Frontiers in molecular neuroscience, 346.

Western Blot: Mouse
PMID	Dilution	Publication
29238610	1:1000	Chen, J, et al. 2017. A role for ErbB signaling in the induction of reactive astrogliosis. Cell discovery, 17044.

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Anti-Profilin (Tyr-129), Phosphospecific Antibody

SKU: PP4751

Product Details

Product Specific References for Applications and Species

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