Anti-Pre-Pro-Vasopressin Antibody

Name: Anti-Pre-Pro-Vasopressin Antibody
Brand: PhosphoSolutions
SKU: 2070-VASO
Price: 430.0 USD
Availability: InStock

1 Citation

Our Pre-Pro-Vasopressin rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects Pre-Pro-Vasopressin and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Western blot of mouse whole brain lysate showing specific immunolabeling of the ~20 kDa uncleaved pre-pro vasopressin protein.

Immunofluorescence of healthy human kidney. The antibody specifically labels pre-pro-vasopressin (white) in the collecting ducts (DBA, red), but not in the proximal tubules (LTL, green) of human kidney. DNA is labeled with DAPI (blue). Image kindly provided by JP Arroyo, Vanderbilt University.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

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from PhosphoSolutions Datasheet

Trial Size

Pooled Serum

SKU: 2070-VASO

Size: 100 µL

Variant

Price:

$430

Quantity:

Product Details

Target

Pre-Pro-Vasopressin

Target Description

Vasopressin is a nine amino-acid peptide hormone that plays a key role in water and blood pressure homeostasis (Qureshi S., et al 2014). Vasopressin is the end-product of a highly processed 164 amino acid pre-pro-peptide. Processing of the vasopressin pre-pro-peptide results in three distinct peptides with a 1:1:1 ratio: vasopressin, neurophysin II, and copeptin (Arroyo J.P. et al, 2021). Vasopressin is the biologically active hormone, neurophysin II is a carrier molecule for vasopressin, and copeptin is the c-terminal glycosylated end-product. Vasopressin has been thought to be primarily made in the brain, and the sole source of vasopressin stimulating vasopressin V2 receptors in the kidney until recent studies (Arroyo JP, et al 2022). This antibody specially detects uncleaved pre-pro-vasopressin, accurately identifying locally produced vasopressin versus peripheral uptake of hypothalamic vasopressin or related peptides (Arroyo J.P. et al, 2022). This key research tool aided the Arroyo group in discovering immunoreactive vasopressin outside of the brain by recognizing localized vasopressin in human and mouse kidney, specifically in the distal nephron.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

AVP

Molecular Weight

20 kDa

Antigen

Synthetic peptide corresponding to key amino acid residues surrounding the cleavage sites for the human vasopressin precursor peptide, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Non-Human Primate

Antibody Registry ID

AB_2927668

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification on a column made with the peptide used as antigen.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for locally produced levels of the ~20 kDa uncleaved pre-pro-vasopressin protein. Immunolabeling of the uncleaved pre-pro-vasopressin protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ADH antibody, Antidiuretic hormone antibody, Arginine vasopressin neurophysin II antibody, ARVP antibody, AVP antibody, AVP NPII antibody, copeptin antibody, Vasopressin neurophysin II copeptin antibody, VP antibody

UniProt Details

UniProt (Human): P01185
UniProt (Immunogen Species): P01185

NCBI Gene ID

551

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse
Western Blot: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
36326835	1:1000	Arroyo, J.P., et al. 2022. Kidney Collecting Duct Cells Make Vasopressin In Response To NaCl Induced Hypertonicity. JCI Insight, .

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36326835	1:1000	Arroyo, J.P., et al. 2022. Kidney Collecting Duct Cells Make Vasopressin In Response To NaCl Induced Hypertonicity. JCI Insight, .

Western Blot: Mouse
PMID	Dilution	Publication
36326835	1:1000	Arroyo, J.P., et al. 2022. Kidney Collecting Duct Cells Make Vasopressin In Response To NaCl Induced Hypertonicity. JCI Insight, .

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Product Specific Protocols

Western Blot Protocol: Download

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Anti-Pre-Pro-Vasopressin Antibody

Our Pre-Pro-Vasopressin rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects Pre-Pro-Vasopressin and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

SKU: 2070-VASO

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Pre-Pro-Vasopressin Antibody

Product Specific Protocols

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