Name: Anti-PRAS40 (Thr356) Antibody
Brand: PhosphoSolutions
SKU: p204-356
Price: 415.0 USD
Availability: InStock

Our Anti-PRAS40 (Thr356) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila PRAS40 (Thr356) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Drosophila

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

AKT1S1

Molecular Weight

60 kDa

Go to Full Product Details

Western blot of Drosophila lysate showing specific immunolabeling of the ~60 kDa PRAS40 protein phosphorylated at Thr356 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-PRAS40 (Thr356) Antibody

Product Details

Target

PRAS40 (Thr356)

Target Description

PRAS40 (proline-rich Akt/PKB substrate of 40 kDa) acts at the intersection of the Akt- and mTOR-mediated signaling pathways and its phosphorylation by Akt and by mTORC1 results in dissociation of PRAS40 from mTORC1 which may relieve an inhibitory constraint on mTORC1 activity (Wiza et al., 2012). Phosphorylation of PRAS40 by Akt and association with 14-3-3 has also been indicated to be crucial for insulin to stimulate mTOR. (Vander Haar et al., 2007). The primary function of PRAS40 in vivo in Drosophila has been shown to regulate TORC1 activity, and not to act as a downstream target and effector of TORC1 (Pallares et al., 2012).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

AKT1S1

Molecular Weight

60 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 of Drosophila PRAS40, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Drosophila

Antibody Registry ID

AB_2492214

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~60 kDa PRAS40 protein phosphorylated at Thr356. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr356

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

40 kDa proline rich AKT substrate antibody, 40 kDa proline-rich AKT substrate antibody, AKT1 S1 antibody, AKT1 substrate 1 (proline rich) antibody, AKT1 substrate 1 antibody, AKT1S 1 antibody, AKT1S1 antibody, AKTS1_HUMAN antibody, Lobe antibody, MGC2865 antibody, PRAS 40 antibody, PRAS antibody, PRAS40 antibody, Proline rich akt substrate antibody, Proline rich Akt substrate 40 kDa antibody, Proline-rich AKT1 substrate 1 antibody

UniProt Details

UniProt (Immunogen Species): Q7JXA2

Product Specific Protocols

Western Blot Protocol: Download

General References

Wiza, C., Nascimento, E.B. and Ouwens, D.M., 2012. Role of PRAS40 in Akt and mTOR signaling in health and disease. American Journal of Physiology-Endocrinology and Metabolism, 302(12), pp.E1453-E1460. PMID: 22354785
Vander Haar, E., Lee, S.I., Bandhakavi, S., Griffin, T.J. and Kim, D.H., 2007. Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40. Nature Cell Biology, 9(3), p.316. PMID: 17277771
Pallares-Cartes, C., Cakan-Akdogan, G. and Teleman, A.A., 2012. Tissue-specific coupling between insulin/IGF and TORC1 signaling via PRAS40 in Drosophila. Developmental Cell, 22(1), pp.172-182. PMID: 22264732

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Anti-PRAS40 (Thr356) Antibody

Our Anti-PRAS40 (Thr356) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila PRAS40 (Thr356) and is antigen affinity purified from pooled serum. It is great for use in WB.