Our Anti-PRAS40 (Thr356) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila PRAS40 (Thr356) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of Drosophila lysate showing specific immunolabeling of the ~60 kDa PRAS40 protein phosphorylated at Thr356 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PRAS40 (proline-rich Akt/PKB substrate of 40 kDa) acts at the intersection of the Akt- and mTOR-mediated signaling pathways and its phosphorylation by Akt and by mTORC1 results in dissociation of PRAS40 from mTORC1 which may relieve an inhibitory constraint on mTORC1 activity (Wiza et al., 2012). Phosphorylation of PRAS40 by Akt and association with 14-3-3 has also been indicated to be crucial for insulin to stimulate mTOR. (Vander Haar et al., 2007). The primary function of PRAS40 in vivo in Drosophila has been shown to regulate TORC1 activity, and not to act as a downstream target and effector of TORC1 (Pallares et al., 2012).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
AKT1S1
60 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 of Drosophila PRAS40, conjugated to keyhole limpet hemocyanin (KLH).
Drosophila
AB_2492214
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~60 kDa PRAS40 protein phosphorylated at Thr356. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Thr356
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Wiza, C., Nascimento, E.B. and Ouwens, D.M., 2012. Role of PRAS40 in Akt and mTOR signaling in health and disease. American Journal of Physiology-Endocrinology and Metabolism, 302(12), pp.E1453-E1460. PMID: 22354785
Vander Haar, E., Lee, S.I., Bandhakavi, S., Griffin, T.J. and Kim, D.H., 2007. Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40. Nature Cell Biology, 9(3), p.316. PMID: 17277771
Pallares-Cartes, C., Cakan-Akdogan, G. and Teleman, A.A., 2012. Tissue-specific coupling between insulin/IGF and TORC1 signaling via PRAS40 in Drosophila. Developmental Cell, 22(1), pp.172-182. PMID: 22264732
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