Name: Anti-Potassium Channel, Voltage-Gated, Kv3.1 Subunit (Ser503) Antibody
Brand: PhosphoSolutions
SKU: p1550-503
Price: 415.0 USD
Availability: InStock

Our Anti-Potassium Channel, Voltage-Gated, Kv3.1 Subunit (Ser503) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects hamster, mouse and rat Potassium Channel, Voltage-Gated, Kv3.1 Subunit (Ser503) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

KCNC1

Molecular Weight

100 kDa

Go to Full Product Details

Immunostaining of Chinese Hamster Overy (CHO) cells stably transfected with KV3.1b gene with the phospho-Ser503 Kv3.1 subunit antibody (cat. p1550-503, green, 1:400). The image on the right shows cells that have been treated with the protein kinase C activator PMA (500nM) while the control cells are on the left.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Potassium Channel, Voltage-Gated, Kv3.1 Subunit (Ser503) Antibody

Product Details

Target

Potassium Channel, Voltage-Gated, Kv3.1 Subunit (Ser503)

Target Description

Voltage-gated K+ channels are important determinants of neuronal membrane excitability. Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types (Maletic-Savatic et al., 1995; Pongs, 1999; Blaine and Ribera, 1998; Burger and Ribera, 1996). The Kv3.1 potassium channel is expressed at high levels in neurons that characteristically fire rapid trains of action potentials (Gan et al., 1999). Particularly high levels of this channel are found in neurons of the auditory brainstem. These neurons appear to participate in neural circuits that determine the intensity and timing of auditory stimuli and use this information to determine the location of sounds in space (von Hehn et al., 2004).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

KCNC1

Molecular Weight

100 kDa

Antigen

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser503 of the rat voltage-gated potassium channel Kv3.1, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2492212

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:400-1:1000
ICC: 1:400

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~100 kDa Kv3.1 voltage-gated potassium channel protein phosphorylated at Ser503.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser503

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

C230009H10Rik antibody, FLJ41162 antibody, FLJ42249 antibody, FLJ43491 antibody, Kcnc1 antibody, KCNC1 antibody, KCNC1_HUMAN antibody, Kcr2 1 antibody, KShIIIB antibody, Kv3.1 antibody, Kv4 antibody, MGC129855 antibody, NGK2 antibody, Potassium voltage-gated channel subfamily C member 1 antibody, Shaw antibody, Voltage gated potassium channel antibody, Voltage gated potassium channel subunit Kv3.1 antibody, Voltage-gated potassium channel subunit Kv3.1 antibody, Voltage-gated potassium channel subunit Kv4 antibody

UniProt Details

UniProt (Immunogen Species): P25122

NCBI Gene ID

25327

Product Specific References for Applications and Species

Immunocytochemistry: Hamster
Immunohistochemistry: Mouse | Rat
Western Blot: Hamster | Mouse | Rat

Immunocytochemistry: Hamster
PMID	Dilution	Publication
16595659	1:400	Song, P., et al. 2006. Modulation of Kv3. 1b potassium channel phosphorylation in auditory neurons by conventional and novel protein kinase C isozymes. Journal of Biological Chemistry, 281(22), pp.15582-15591.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
30804492	not listed	Sagi, Y., et al. 2019. Emergence of 5-HT5A signaling in parvalbumin neurons mediates delayed antidepressant action. Molecular Psychiatry, p.1.

Immunohistochemistry: Rat
PMID	Dilution	Publication
16595659	1:400	Song, P., et al. 2006. Modulation of Kv3. 1b potassium channel phosphorylation in auditory neurons by conventional and novel protein kinase C isozymes. Journal of Biological Chemistry, 281(22), pp.15582-15591.

Western Blot: Hamster
PMID	Dilution	Publication
16595659	not listed	Song, P., et al. 2006. Modulation of Kv3. 1b potassium channel phosphorylation in auditory neurons by conventional and novel protein kinase C isozymes. Journal of Biological Chemistry, 281(22), pp.15582-15591.

Western Blot: Hamster
PMID	Dilution	Publication
30804492	not listed	Sagi, Y., et al. 2019. Emergence of 5-HT5A signaling in parvalbumin neurons mediates delayed antidepressant action. Molecular Psychiatry, p.1.

Western Blot: Rat
PMID	Dilution	Publication
16595659	not listed	Song, P., et al. 2006. Modulation of Kv3. 1b potassium channel phosphorylation in auditory neurons by conventional and novel protein kinase C isozymes. Journal of Biological Chemistry, 281(22), pp.15582-15591.

Product Specific Protocols

Potassium Channel, Voltage-Gated, Kv3.1 Subunit Ser503 ICC Protocol: Download

Western Blot Protocol: Download

Recover password

Create my account

Anti-Potassium Channel, Voltage-Gated, Kv3.1 Subunit (Ser503) Antibody