Name: Anti-Polo-Like Kinase (Thr210) Antibody
Brand: PhosphoSolutions
SKU: p202-210
Price: 415.0 USD
Availability: InStock

Our Anti-Polo-Like Kinase (Thr210) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Polo-Like Kinase (Thr210) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Rat

Expected Reactivity

Bovine, Canine, Non-Human Primate, Mouse, Xenopus, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

PLK1

Molecular Weight

66 kDa

Go to Full Product Details

Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 kDa PLK protein phosphorylated at Thr210 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Polo-Like Kinase (Thr210) Antibody

Product Details

Target

Polo-Like Kinase (Thr210)

Target Description

Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK’s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser-137 and Thr-210 (van de Weerdt et al., 2005).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

PLK1

Molecular Weight

66 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 of human PLK1, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Rat

Expected Reactivity

Bovine, Canine, Non-Human Primate, Mouse, Xenopus, Zebrafish

Antibody Registry ID

AB_2492209

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~66 kDa PLK phosphorylated at Thr210. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr210

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Cell cycle regulated protein kinase antibody, PLK 1 antibody, PLK antibody, PLK-1 antibody, plk1 antibody, PLK1_HUMAN antibody, Polo like kinase 1 antibody, Polo-like kinase 1 antibody, Serine/threonine protein kinase 13 antibody, Serine/threonine protein kinase PLK1 antibody, Serine/threonine-protein kinase 13 antibody, Serine/threonine-protein kinase PLK1 antibody, STPK 13 antibody, STPK13 antibody

UniProt Details

UniProt (Human): P53350
UniProt (Immunogen Species): P53350

NCBI Gene ID

5347

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
15713655	not listed	van de Weerdt, BC., et al. 2005. Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1. Molecular and Cellular Biology, 25(5), pp.2031-2044.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Polo-Like Kinase (Thr210) Antibody

Our Anti-Polo-Like Kinase (Thr210) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Polo-Like Kinase (Thr210) and is antigen affinity purified from pooled serum. It is great for use in WB.