Our Anti-Polo-Like Kinase (Thr210) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Polo-Like Kinase (Thr210) and is antigen affinity purified from pooled serum. It is great for use in WB.
Primary Antibody
Human, Rat
Bovine, Canine, Non-Human Primate, Mouse, Xenopus, Zebrafish
WB
Rabbit
Antigen Affinity Purified from Pooled Serum
PLK1
66 kDa

Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 kDa PLK protein phosphorylated at Thr210 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
Product Specific References for Applications and Species
- Western Blot: Human
Western Blot: Human | ||
PMID | Dilution | Publication |
15713655 | not listed | van de Weerdt, BC., et al. 2005. Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1. Molecular and Cellular Biology, 25(5), pp.2031-2044. |
Product Specific Protocols
- Western Blot Protocol: Download