Name: Anti-Polo-Like Kinase Kinase (Ser482,486,490) Antibody
Brand: PhosphoSolutions
SKU: p200-48260
Price: 415.0 USD
Availability: InStock

Our Anti-Polo-Like Kinase Kinase (Ser482,486,490) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat and Xenopus Polo-Like Kinase Kinase (Ser482,486,490) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Xenopus

Expected Reactivity

Human, Mouse, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

PLKK1

Molecular Weight

120 kDa

Go to Full Product Details

Western blot of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units overnight).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Polo-Like Kinase Kinase (Ser482,486,490) Antibody

Product Details

Target

Polo-Like Kinase Kinase (Ser482,486,490)

Target Description

Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser-482, Ser-486 and Ser-490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser-482,486,490 in PLKK.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

PLKK1

Molecular Weight

120 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 of Xenopus polo-like kinase kinase (PLKK), conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Xenopus

Expected Reactivity

Human, Mouse, Zebrafish

Antibody Registry ID

AB_2492210

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~120k PLKK protein phosphorylated at Ser482,486,490.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser482,486,490

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Polo like kinase kinase 1 antibody, Serine/threonine kinase 10 antibody, stk10 a antibody, xPlkk1 antibody

UniProt Details

UniProt (Human): P53350
UniProt (Immunogen Species): B7ZR30

NCBI Gene ID

380481

Product Specific Protocols

Western Blot Protocol: Download

General References

Erikson, E., Haystead, T.A., Qian, Y.W. and Maller, J.L., 2004. A feedback loop in the polo-like kinase activation pathway. Journal of Biological Chemistry, 279(31), pp.32219-32224. PMID: 15166215
Kumagai, A. and Dunphy, W.G., 1996. Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts. Science, 273(5280), pp.1377-1380. PMID: 8703070
Liu, J., Lewellyn, A.L., Chen, L.G. and Maller, J.L., 2004. The polo box is required for multiple functions of Plx1 in mitosis. Journal of Biological Chemistry, 279(20), pp.21367-21373. PMID: 15016807
van Vugt, M.A., van de Weerdt, B.C., Vader, G., Janssen, H., Calafat, J., Klompmaker, R., Wolthuis, R.M. and Medema, R.H., 2004. Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis. Journal of biological chemistry, 279(35), pp.36841-36854. PMID: 15210710

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Anti-Polo-Like Kinase Kinase (Ser482,486,490) Antibody

Our Anti-Polo-Like Kinase Kinase (Ser482,486,490) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat and Xenopus Polo-Like Kinase Kinase (Ser482,486,490) and is antigen affinity purified from pooled serum. It is great for use in WB.