Anti-Plexin A1 (Sema Domain) Antibody (M535)

Name: Anti-Plexin A1 (Sema Domain) Antibody
Brand: PhosphoSolutions
SKU: PM5351
Price: 340.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M535

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Format

Antigen Affinity Purified

Gene Name

PLXNA1

Molecular Weight

220

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Immunocytochemical labeling of Plexin A1 in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Plexin A1 (PM5351). The antibody was detected using goat anti-mouse DyLight® 594.

Western blots showing mouse recombinant Plexin-A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin-A1 (PP1301) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (PM5351) at 1:250 (lane 3) and 1:1000 (lane 4).

Volume: 100 µL

Variant

Price:

$340

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Product Details

Target

Plexin A1 (Sema Domain)

Target Description

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. This region in Plexin-A1 binds the RhoGTPases, Rnd1 and RhoD. Recruitment of Rnd1 has been implicated in the cytoskeletal collapse that occurs after semaphorin-mediated activation of Plexin-A1, while RhoD may block this collapsing activity through interaction with the cytoplasmic region of Plexin-A1. The expression of Plexin-A1, along with the co-receptor Neuropilin-1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.<

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M535

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

PLXNA1

Molecular Weight

220

Antigen

Clone M535 was generated from a Plexin-A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the N-terminal half of the semaphorin domain in human Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
IHC: 1:100
ICC: 1:100
IP: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 220 kDa* protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of neonatal mouse brain, and detects a recombinant mouse Plexin A1 protein containing the extracellular region.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PLXN1, NOV, Sema3A

UniProt Details

UniProt (Human): Q9UIW2
UniProt (Immunogen Species): Q9UIW2

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Anti-Plexin A1 (Sema Domain) Antibody (M535)

Anti-Plexin A1 (Sema Domain) Antibody
SKU: PM5351

Bulk Order Anti-Plexin A1 (Sema Domain) Antibody

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Anti-Plexin A1 (Sema Domain) Antibody (M535)

Anti-Plexin A1 (Sema Domain) Antibody SKU: PM5351

Bulk Order Anti-Plexin A1 (Sema Domain) Antibody

Product Details

Anti-Plexin A1 (Sema Domain) Antibody
SKU: PM5351