Anti-PKCθ (N-terminal region) Antibody (M217)

Name: Anti-PKCθ (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: PM2171
Price: 340.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M217

Applications

ICC, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

PRKCQ

Molecular Weight

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Immunocytochemical labeling of PKCθ in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal PKCθ (N-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.

Western blot analysis of PKCθ in human Jurkat cell lysate. The blot was probed with anti-PKCθ at 1:250 (lane 1) and 1:1000 (lane 2).

Volume: 100 µL

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Product Details

Target

PKCθ (N-terminal region)

Target Description

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. The phosphorylation of multiple sites in PKCs regulates their activity

Format

Protein A Purified

Clonality

Monoclonal

Clone

M217

Isotype

IgG2a

Applications

ICC, WB

Host Species

Mouse

Gene Name

PRKCQ

Molecular Weight

Antigen

Clone (M217) was generated from a recombinant mouse PKCθ protein that included amino acids residues in the N-terminal region. This sequence is conserved in human and rat PKCθ, and has low homology to other PKC family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects a 79 kDa* protein corresponding to the molecular mass of PKCθ on SDS-PAGE immunoblots of human Jurkat cell lysates.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PKC theta

UniProt Details

UniProt (Immunogen Species): A6H667

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Anti-PKCθ (N-terminal region) Antibody (M217)

Anti-PKCθ (N-terminal region) Antibody
SKU: PM2171

Bulk Order Anti-PKCθ (N-terminal region) Antibody

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Anti-PKCθ (N-terminal region) Antibody (M217)

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Anti-PKCθ (N-terminal region) Antibody
SKU: PM2171