Anti-Phosphoserine/threonine Antibody (M380A/M380B)

Name: Anti-Phosphoserine/threonine Antibody
Brand: PhosphoSolutions
SKU: PM3801
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M380A/M380B

Applications

ICC, IP, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

N/A

Molecular Weight

N/A

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Immunocytochemical labeling of phosphoserine and phosphothreonine in control and calyculin A-treated A431 cells. The cells were labeled with mouse monoclonal anti-Phosphoserine/threonine (PM3801) and rabbit polyclonal anti-Phosphoserine/threonine (PP2551), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).

Volume: 100 µL

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Product Details

Target

Anti-Phosphoserine/threonine

Target Description

Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M380A/M380B

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

N/A

Molecular Weight

N/A

Antigen

PM3801 Phosphoserine/threonine is a mix of two clones: Clone M380A was generated from a phosphothreonine synthetic peptide (coupled to carrier protein) and Clone M380B was generated.from a phosphoserine synthetic peptide (coupled to carrier protein).

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:50
IP: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

pSer/Thr

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Phosphoser/thr mAb

UniProt Details

UniProt (Immunogen Species): N/A

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Anti-Phosphoserine/threonine Antibody (M380A/M380B)

Anti-Phosphoserine/threonine Antibody
SKU: PM3801

Bulk Order Anti-Phosphoserine/threonine Antibody

Product Details

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Anti-Phosphoserine/threonine Antibody (M380A/M380B)

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Product Details

Anti-Phosphoserine/threonine Antibody
SKU: PM3801