Name: Anti-Peripherin Antibody
Brand: PhosphoSolutions
SKU: 1631-PER
Price: 360.0 USD
Availability: InStock

Our Anti-Peripherin primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Peripherin and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Total IgY fraction

Gene Name

PRPH

Molecular Weight

57 kDa

Go to Full Product Details

Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin (cat. 1631-PER, green, 1:5000) and anti-vimentin (red). The blue is DAPI staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.

Immunoflourescence of cultured rat neurons and glia showing peripherin (cat. 1631-PER, green, 1:500) and alpha-internexin (red). The blue stain is nuclear DNA. Cells expressing both peripherin and alpha-internexin appear yellow.

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~57 kDa peripherin protein.

Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin (cat. 1631-PER, green, 1:5000) and anti-vimentin (red). The blue is DAPI staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.

Volume: 100 µL

Variant

Price:

$360

Quantity:

Bulk Order Anti-Peripherin Antibody

Product Details

Target

Peripherin

Target Description

Peripherin is a ~57 kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. In contrast to the neurofilaments, peripherin is strongly up-regulated after nerve injury (1). Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors (2). Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes (3). Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.

Format

Total IgY fraction

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

PRPH

Molecular Weight

57 kDa

Antigen

Recombinant rat peripherin expressed in and purified from E. coli.

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep

Antibody Registry ID

AB_2492208

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Total IgY fraction

Buffer

PBS + 10 mM NaN3.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:500-1:2000
ICC: 1:500-1:5000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~57 kDa peripherin protein. This antibody performs well on aldehyde fixed tissues.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

NEF 4 antibody, NEF4 antibody, Neurofilament 4 (57kD) antibody, Neurofilament 4 antibody, Perf antibody, PERI_HUMAN antibody, Peripherin antibody, PRPH 1 antibody, prph antibody, PRPH1 antibody

UniProt Details

UniProt (Human): P41219
UniProt (Immunogen Species): P21807

NCBI Gene ID

3414

Product Specific Protocols

Perpherin Cultured Rat Neurons and Glia ICC Protocol: Download

Perpherin Mixed Fibroblasts and PC12 Cells ICC Protocol: Download

Western Blot Protocol: Download

General References

Terao E, Janssens S, van den Bosch de Aquilar P, Portier M, Klosen P (2000) In vivo expression of the intermediate filament peripherin in rat motorneurons: modulation by inhibitory and stimulatory signals. Neuroscience 101(3):679-88. PMID: 11113316
Roberson J, Doroudchi MM, Nguyen MD, Durham, HD, Shaw G, Julien JP, Mushynski WE. (2003) A neurotoxic peripherin splice variant in a mouse model of ALS. Journal of Cell Biology 160(6):939-49. PMID: 12642616
Boitard C, Villa MC, Becourt C, Gia HP, Huc C, Sempe P, Portier MM, Bach JF (1992) Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products. Proc Natl Acad Sci USA 89(1):172-6. PMID: 1729686

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Anti-Peripherin Antibody

Our Anti-Peripherin primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Peripherin and is total IgY fraction. It is great for use in WB, IHC, ICC.