Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin (cat. 1631-PER, green, 1:5000) and anti-vimentin (red). The blue is DAPI staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.
Peripherin is a ~57 kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. In contrast to the neurofilaments, peripherin is strongly up-regulated after nerve injury (1). Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors (2). Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes (3). Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.
Total IgY fraction
ICC, IHC, WB
Recombinant rat peripherin expressed in and purified from E. coli.
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Terao E, Janssens S, van den Bosch de Aquilar P, Portier M, Klosen P (2000) In vivo expression of the intermediate filament peripherin in rat motorneurons: modulation by inhibitory and stimulatory signals. Neuroscience 101(3):679-88. PMID: 11113316
Roberson J, Doroudchi MM, Nguyen MD, Durham, HD, Shaw G, Julien JP, Mushynski WE. (2003) A neurotoxic peripherin splice variant in a mouse model of ALS. Journal of Cell Biology 160(6):939-49. PMID: 12642616
Boitard C, Villa MC, Becourt C, Gia HP, Huc C, Sempe P, Portier MM, Bach JF (1992) Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products. Proc Natl Acad Sci USA 89(1):172-6. PMID: 1729686
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