Western blot analysis of human A431 cells treated with Calyculin A (100 nm) for 30 min (lanes 1-6). The blot was treated with lambda phosphatase (lanes 2, 4, & 6), then probed with rabbit polyclonal anti-Paxillin (C-terminal; PP1161) (lanes 1 & 2), anti-phospho-Paxillin (Thr-538; PP4491) (lanes 3 & 4), and mouse monoclonal anti-Paxillin (PM1071) (lanes 5 & 6).
Anti-Paxillin (Thr-538), Phosphospecific Antibody
Bulk Order Anti-Paxillin (Thr-538), Phosphospecific Antibody
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at tyrosine, threonine, and serine sites. Serine and threonine phosphorylation of paxillin occur in response to growth-factor activation, PKC activators, and fibronectins. Phosphorylation of Ser-85, Ser-178, and Thr-538 may be important sites for regulating paxillin activity. Paxillin phosphorylation of Thr-538 occurs in response to TPA-activated PKCs in vitro, and this phosphorylation may contribute to dissolution of the actin cytoskeleton and redistribution of LFA-1 integrins in vivo.
Antigen Affinity Purified
Phospho-Paxillin (Thr-538) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 538 from human paxillin a. This sequence is highly conserved in rat and mouse paxillin and is also found in the other paxillin isoforms (b & g).
Chicken, Frog, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.