Anti-Paxillin (C-terminal) Antibody

Name: Anti-Paxillin (C-terminal) Antibody
Brand: PhosphoSolutions
SKU: PP1161
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

PXN

Molecular Weight

Go to Full Product Details

Western blot analysis of A431 cells. The blot was probed with rabbit polyclonal anti-Paxillin (PP1161) at 1:500 & 1:2000 (lanes 1 & 2) or with mouse monoclonal anti-Paxillin (PM1071) at 1:2000 & 1:5000 (lanes 3 & 4).

Volume: 100 µL

Variant

Price:

$255

Quantity:

Bulk Order Anti-Paxillin (C-terminal) Antibody

Product Details

Target

Paxillin (C-terminal)

Target Description

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

PXN

Molecular Weight

Antigen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Paxillin. This peptide sequence is highly conserved in rat and mouse paxillins, and is found in all three paxillin isoforms (α, β,and γ).

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

UniProt Details

UniProt (Human): P49023
UniProt (Immunogen Species): P49023

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Paxillin (C-terminal) Antibody