Our Anti-Parkin (Ser378) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Parkin (Ser378) and is antigen affinity purified. It is great for use in WB.
Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S378 mutant(Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser378.
Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substantia nigra (Jenner et al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser-378 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).
Antigen Affinity Purified from Pooled Serum
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser378 of human Parkin, conjugated to keyhole limpet hemocyanin (KLH).
Bovine, Non-Human Primate
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Specific for the ~52 kDa parkin protein phosphorylated at Ser378. Immunolabeling of the parkin band is absent in parkin S378 mutants.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.