Name: Anti-PAK-1,2,3 (Thr402) Antibody
Brand: PhosphoSolutions
SKU: p187-402
Price: 415.0 USD
Availability: InStock

Our Anti-PAK-1,2,3 (Thr402) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat PAK-1,2,3 (Thr402) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Human

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

PAK1-3

Molecular Weight

68/70 kDa

Go to Full Product Details

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68 kDa to ~70 kDa PAK protein phosphorylated at Ser402 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-PAK-1,2,3 (Thr402) Antibody

Product Details

Target

PAK-1,2,3 (Thr402)

Target Description

In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: α-PAK (also known as PAK-1) and β-PAK (also known as PAK-3) are mostly brain-specific, while γ-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr-402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

PAK1-3

Molecular Weight

68/70 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 of rat p21 activated kinase 2 (PAK-2), conjugated to keyhole limpet hemocyanin (KLH). The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1.

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Human

Antibody Registry ID

AB_2492200

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~68 kDa PAK protein phosphorylated at Thr402. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr402

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Alpha PAK antibody, Beta PAK antibody, bPAK antibody, CDKN1A antibody, Gamma PAK antibody, hPAK3 antibody, MRX30 antibody, MRX47 antibody, Oligophrenin 3 antibody, OPHN3 antibody, P21 (CDKN1A) activated kinase 2 antibody, P21 (CDKN1A) activated kinase 3 antibody, p21 activated kinase 1 antibody, p21 activated kinase 2 antibody, p21 activated kinase 3 antibody, P21 protein (Cdc42/Rac) activated kinase 1 antibody, P21 protein (Cdc42/Rac) activated kinase 2 antibody, P21 protein (Cdc42/Rac) activated kinase 3 antibody, P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog yeast) antibody, P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody, P58 antibody, P65 PAK antibody, PAK 2 antibody, PAK 3 antibody, PAK1 antibody, PAK2 antibody, PAK3 antibody, PAK3beta antibody, PAK65 antibody, PAKalpha antibody, PAKgamma antibody, S6/H4 kinase antibody, Serine/threonine protein kinase PAK 1 antibody, Serine/threonine protein kinase PAK 2 antibody, Serine/threonine protein kinase PAK 3 antibody, Serine/threonine protein kinase PAK1 antibody, Serine/threonine protein kinase PAK2 antibody, Serine/threonine protein kinase PAK3 antibody, STE20 homolog yeast antibody

UniProt Details

UniProt (Human): Q13153
UniProt (Immunogen Species): P35465

NCBI Gene ID

29431

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
23629625	not listed	Nie, J., et al. 2013. SAD-A potentiates glucose-stimulated insulin secretion as a mediator of glucagon-like peptide 1 response in pancreatic β cells. Molecular and cellular biology, 33(13), pp.2527-2534.
22669945	not listed	Nie, J., et al. 2012. Synapses of Amphids Defective (SAD-A) Kinase Promotes Glucose-stimulated Insulin Secretion through Activation of p21-activated Kinase (PAK1) in Pancreatic β-Cells. J. Biol. Chem. 287:26435-26444.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-PAK-1,2,3 (Thr402) Antibody

Our Anti-PAK-1,2,3 (Thr402) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat PAK-1,2,3 (Thr402) and is antigen affinity purified from pooled serum. It is great for use in WB.