Our Anti-PAK-1,2,3 (Thr402) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat PAK-1,2,3 (Thr402) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68 kDa to ~70 kDa PAK protein phosphorylated at Ser402 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: α-PAK (also known as PAK-1) and β-PAK (also known as PAK-3) are mostly brain-specific, while γ-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr-402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
PAK1-3
68/70 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 of rat p21 activated kinase 2 (PAK-2), conjugated to keyhole limpet hemocyanin (KLH). The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1.
Rat
Bovine, Canine, Human
AB_2492200
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~68 kDa PAK protein phosphorylated at Thr402. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Thr402
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Alpha PAK antibody, Beta PAK antibody, bPAK antibody, CDKN1A antibody, Gamma PAK antibody, hPAK3 antibody, MRX30 antibody, MRX47 antibody, Oligophrenin 3 antibody, OPHN3 antibody, P21 (CDKN1A) activated kinase 2 antibody, P21 (CDKN1A) activated kinase 3 antibody, p21 activated kinase 1 antibody, p21 activated kinase 2 antibody, p21 activated kinase 3 antibody, P21 protein (Cdc42/Rac) activated kinase 1 antibody, P21 protein (Cdc42/Rac) activated kinase 2 antibody, P21 protein (Cdc42/Rac) activated kinase 3 antibody, P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog yeast) antibody, P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody, P58 antibody, P65 PAK antibody, PAK 2 antibody, PAK 3 antibody, PAK1 antibody, PAK2 antibody, PAK3 antibody, PAK3beta antibody, PAK65 antibody, PAKalpha antibody, PAKgamma antibody, S6/H4 kinase antibody, Serine/threonine protein kinase PAK 1 antibody, Serine/threonine protein kinase PAK 2 antibody, Serine/threonine protein kinase PAK 3 antibody, Serine/threonine protein kinase PAK1 antibody, Serine/threonine protein kinase PAK2 antibody, Serine/threonine protein kinase PAK3 antibody, STE20 homolog yeast antibody
Nie, J., et al. 2013. SAD-A potentiates glucose-stimulated insulin secretion as a mediator of glucagon-like peptide 1 response in pancreatic β cells. Molecular and cellular biology, 33(13), pp.2527-2534.
Nie, J., et al. 2012. Synapses of Amphids Defective (SAD-A) Kinase Promotes Glucose-stimulated Insulin Secretion through Activation of p21-activated Kinase (PAK1) in Pancreatic β-Cells. J. Biol. Chem. 287:26435-26444.
