Name: Anti-p70 S6 Kinase (Ser398) Antibody
Brand: PhosphoSolutions
SKU: p1705-398
Price: 415.0 USD
Availability: InStock

Our Anti-p70 S6 Kinase (Ser398) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila p70 S6 Kinase (Ser398) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Drosophila

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

S6K

Molecular Weight

70 kDa

Go to Full Product Details

Western blot of Drosophila S2 cell lysate showing specific labeling of the ~70 kDa p70 S6K protein phosphorylated at Thr398 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-p70 S6 Kinase (Ser398) Antibody

Product Details

Target

p70 S6 Kinase (Ser398)

Target Description

p70 S6 kinase (p70 S6K) is activated in a signaling pathway that includes mTOR and is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (Xio et al., 2009). p70 S6K is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains and subsequently phosphorylates specifically ribosomal protein S6 (Saitoh et al., 2002). Phosphorylation of Thr-229 in the catalytic domain and Thr-389 in the linker domain are most critical for kinase function. Inhibition of p70 activity inhibits the entry into S phase of the cell cycle and exhibits cell cycle arrest at G0/G1 phase, suggesting that the activation of p70 S6k plays an obligatory role in mediating mitogenic signals during cell activation (Xio et al., 2009).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

S6K

Molecular Weight

70 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr398 of Drosophila p70 S6K protein, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Drosophila

Antibody Registry ID

AB_2629470

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~70 kDa p70 S6K protein phosphorylated at Thr398. Immunolabeling is blocked by preadsorption with the Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser398

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

70 kDa ribosomal protein S6 kinase 2 antibody, EC 2.7.11.1 antibody, KS6B2_HUMAN antibody, p70 beta antibody, p70 ribosomal S6 kinase beta antibody, p70 S6 kinase beta antibody, p70 S6K-beta antibody, p70 S6KB antibody, p70 S6Kbeta antibody, p70(S6K) beta antibody, p70-beta antibody, p70-S6K 2 antibody, P70S6K2 antibody, p70S6Kb antibody, Ribosomal protein S6 kinase 70kDa polypeptide 2 antibody, Ribosomal protein S6 kinase B2 antibody, Ribosomal protein S6 kinase beta 2 antibody, Ribosomal protein S6 kinase beta-2 antibody, Rps6kb2 antibody, S6 kinase related kinase antibody, S6 kinase-related kinase antibody, S6K beta 2 antibody, S6K beta antibody, S6K-beta antibody, S6K-beta-2 antibody, S6K2 antibody, Serine/threonine protein kinase 14 beta antibody, Serine/threonine-protein kinase 14B antibody, SRK antibody, STK14B antibody

UniProt Details

UniProt (Immunogen Species): H1ZYE3

Product Specific References for Applications and Species

Western Blot: Drosophila

Western Blot: Drosophila
PMID	Dilution	Publication
34795214	1:1000	Strassburger, K., et al. 2021. Ecdysone regulates Drosophila wing disc size via a TORC1 dependent mechanism. Nature communications, 12(1), pp.1-12.
26988032	not listed	Tsokanos, F.F., et al. 2016. eIF4A inactivates TORC1 in response to amino acid starvation. The EMBO Journal, 35(10), pp.1058-1076.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-p70 S6 Kinase (Ser398) Antibody

Our Anti-p70 S6 Kinase (Ser398) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila p70 S6 Kinase (Ser398) and is antigen affinity purified from pooled serum. It is great for use in WB.