Name: Anti-p62 (Thr269, Ser272) Antibody
Brand: PhosphoSolutions
SKU: p196-269
Price: 415.0 USD
Availability: InStock

Our Anti-p62 (Thr269, Ser272) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human p62 (Thr269, Ser272) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SQSTM1

Molecular Weight

62 kDa

Go to Full Product Details

AD293, A375 and MDA-MB-231 cells were treated with DMSO (control), or MG132 (2 μM), and Doramapimod (50 μM), alone or in combination for 14 h. The whole-cell lysates were subjected to western blot analysis with indicated antibodies. Image from publication CC-BY-4.0. PMID: 35840557

Western blot of Jurkat cell lysate showing specific immunolabeling of the ~62 kDa p62 phosphorylated at Thr269/Ser272 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-p62 (Thr269, Ser272) Antibody

Product Details

Target

p62 (Thr269, Ser272)

Target Description

The protein scaffold and signaling regulator p62 (also known as sequestosome1 (SQSTM1)) is important in critical cellular functions, including bone homeostasis, obesity, and cancer, because of its interactions with various signaling intermediaries. p62 is overexpressed in many human cancers and is induced during cell transformation. cdk1 phosphorylates p62 in vitro and in vivo at Thr-269 and Ser-272, which is necessary for the maintenance of appropriate cyclin B1 levels and the levels of cdk1 activity necessary to allow cells to properly enter and exit mitosis (Moscat et al., 2011). The lack of cdk1-mediated phosphorylation of p62 leads to a faster exit from mitosis, translating into enhanced cell proliferation and tumorigenesis in response to Ras-induced transformation (Moscat et al., 2011).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

SQSTM1

Molecular Weight

62 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr269/Ser272 of human p62, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Antibody Registry ID

AB_2492198

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~62 kDa p62 protein phosphorylated at Thr269 and Ser272. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr269, Ser272

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

A170 antibody, DMRV antibody, EBI 3 associated protein of 60 kDa antibody, EBI 3 associated protein p60 antibody, EBI3 associated protein of 60 kDa antibody, EBI3 associated protein p60 antibody, EBI3-associated protein of 60 kDa antibody, EBIAP antibody, FTDALS3 antibody, MGC127197 antibody, ORCA antibody, OSF-6 antibody, Osi antibody, OSIL antibody, Oxidative stress induced like antibody, p60 antibody, p62 antibody, p62B antibody, Paget disease of bone 3 antibody, PDB 3 antibody, PDB3 antibody, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody, Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody, PKC-zeta-interacting protein antibody, Protein kinase C-zeta-interacting protein antibody, Sequestosome 1 antibody, Sequestosome-1 antibody, SQSTM 1 antibody, SQSTM_HUMAN antibody, Sqstm1 antibody, STAP antibody, STONE14 antibody, Ubiquitin binding protein p62 antibody, Ubiquitin-binding protein p62 antibody, ZIP 3 antibody, ZIP antibody, ZIP3 antibody

UniProt Details

UniProt (Human): Q13501
UniProt (Immunogen Species): Q13501

NCBI Gene ID

8878

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
35840557	1:1000	Zhang, C., et al. 2022. Autophagic sequestration of SQSTM1 disrupts the aggresome formation of ubiquitinated proteins during proteasome inhibition. Cell death & disease, 615.
29930081	not listed	Zhang, C., et al. 2018. p38δ MAPK regulates aggresome biogenesis by phosphorylating SQSTM1 in response to proteasomal stress. J Cell Sci, Jul 26;131(14).

Western Blot: Mouse
PMID	Dilution	Publication
26279575	not listed	Linares, J.F., et al. 2015. Amino acid activation of mTORC1 by a PB1-domain-driven kinase complex cascade. Cell reports, 12(8), pp.1339-1352.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-p62 (Thr269, Ser272) Antibody

Our Anti-p62 (Thr269, Ser272) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human p62 (Thr269, Ser272) and is antigen affinity purified from pooled serum. It is great for use in WB.