Name: Anti-p38 MAPK (Thr180/Tyr182) Antibody
Brand: PhosphoSolutions
SKU: p190-1802
Price: 415.0 USD
Availability: InStock

Our Anti-p38 MAPK (Thr180/Tyr182) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse p38 MAPK (Thr180/Tyr182) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Chicken, Non-Human Primate, Rat, Zebrafish

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

MAPK14

Molecular Weight

39 kDa

Go to Full Product Details

Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 (red, 1:250). Photo courtesy of Patsy Ruegg.

Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0’ or 30’ showing the specific immunolabeling of the ~39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-p38 MAPK (Thr180/Tyr182) Antibody

Product Details

Target

p38 MAPK (Thr180/Tyr182)

Target Description

The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress (Johnson and Lapadat, 2002; Shi and Gaestel, 2002). The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003; Ying et al., 2002). Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr-180 and Tyr-182 (Lin et al., 1995).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

MAPK14

Molecular Weight

39 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 of rat p38/MAPK, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Chicken, Non-Human Primate, Rat, Zebrafish

Antibody Registry ID

AB_2492195

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:250

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr180, Tyr182

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CSAID Binding Protein 1 antibody, CSAID binding protein antibody, CSAID-binding protein antibody, Csaids binding protein antibody, CSBP 1 antibody, CSBP 2 antibody, CSBP antibody, CSBP1 antibody, CSBP2 antibody, CSPB1 antibody, Cytokine suppressive anti-inflammatory drug-binding protein antibody, EXIP antibody, MAP kinase 14 antibody, MAP kinase MXI2 antibody, MAP kinase p38 alpha antibody, MAPK 14 antibody, MAPK14 antibody, MAX interacting protein 2 antibody, MAX-interacting protein 2 antibody, Mitogen Activated Protein Kinase 14 antibody, Mitogen activated protein kinase p38 alpha antibody, Mitogen-activated protein kinase 14 antibody, Mitogen-activated protein kinase p38 alpha antibody, MK14_HUMAN antibody, Mxi 2 antibody, MXI2 antibody, p38 ALPHA antibody, p38 antibody, p38 MAP kinase antibody, p38 MAPK antibody, p38 mitogen activated protein kinase antibody, p38ALPHA antibody, p38alpha Exip antibody, PRKM14 antibody, PRKM15 antibody, RK antibody, SAPK2A antibody

UniProt Details

UniProt (Human): Q16539
UniProt (Immunogen Species): P70618

NCBI Gene ID

70496

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
31687975	not listed	Wiley, C.D., et al. 2019. Secretion of leukotrienes by senescent lung fibroblasts promotes pulmonary fibrosis. JCI Insight. 4(24).
28378188	not listed	Perrott, K.M., et al. 2017. Apigenin suppresses the senescence-associated secretory phenotype and paracrine effects on breast cancer cells. GeroScience, pp.1-13.
28264028	not listed	Xu, P., et al. 2017. Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway. PLoS pathogens, 13(3), p.e1006266.
24670723	1:3000	Alspach, E., et al. 2014. p38MAPK plays a crucial role in stromal-mediated tumorigenesis. Cancer discovery, 4(6), pp.716-729.

Western Blot: Mouse
PMID	Dilution	Publication
33811820	not listed	Wiley, C.D., et al. 2021. Oxylipin biosynthesis reinforces cellular senescence and allows detection of senolysis. Cell Metabolism.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-p38 MAPK (Thr180/Tyr182) Antibody

Our Anti-p38 MAPK (Thr180/Tyr182) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse p38 MAPK (Thr180/Tyr182) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.