Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody (M139)

Name: Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: PM1391
Price: 340.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M139

Applications

ICC, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

Mapk14

Molecular Weight

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Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38α (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).

Volume: 100 µL

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$340

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Bulk Order Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody

Product Details

Target

p38 MAP Kinase (Thr-180/Tyr-182)

Target Description

p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (α,β,γ,δ) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. T cells possess an alternative pathway for p38 activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M139

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

Mapk14

Molecular Weight

Antigen

Clone M139 was generated from a phospho-p38α (Thr-180/Tyr-182) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 180 and tyrosine 182 of rat p38α. This peptide sequence is highly conserved in the p38β,g, and d MAPKs, and is identical in human and mouse p38α.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:250

Conjugate

Unconjugated

Specificity

This antibody detects a 38 kDa* protein corresponding to the apparent molecular mass of p38α on SDS-PAGE immunoblots of pervanadate treated human Jurkat and A431 cells, as well as anisomycin treated human HeLa cells.

Post Translational Modification Type

GOmAb/Phosphorylated

Post Translational Modification Residue

Thr-180/Tyr-182

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

MAPK, p38, p38alpha, p38MAPK

UniProt Details

UniProt (Human): Q16539
UniProt (Immunogen Species): P70618

Product Specific Protocols

Western Blot Protocol: Download

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Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody (M139)

Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody
SKU: PM1391

Bulk Order Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody

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Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody (M139)

Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody SKU: PM1391

Bulk Order Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody

Product Details

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Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody
SKU: PM1391