Our Anti-Olig2 (Ser10/13/14) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Olig2 (Ser10/13/14) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~32 kDa form of the Olig2 protein phosphorylated at Ser10,13,14 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding non-phosphopeptide (not shown).
Anti-Olig2 (Ser10/13/14) Antibody
Olig2 is a well conserved bHLH transcription factor that shows both anti-neural functions and pro-neural functions at different stages in the formation of the oligodendrocyte lineage (Sun et al., 2011). Olig2 is expressed in 100% of the human diffuse gliomas irrespective of grade and required for intracranial tumor formation in a genetically relevant model of malignant glioma (Ligon et al., 2004; Ligon et al., 2007). A developmentally regulated triple serine motif at positions 10, 13 and 14 in the amino terminus is well conserved across species ranging from humans to zebrafish and is essential for Olig2 proliferative function in both normal and malignant neural progenitors (Sun et al., 2011). All three serine residues must be mutated to achieve a strong loss-of-function or gain-of-function phenotype, suggesting that the phosphorylation state of Olig2 represents a significant conformational change in the amino terminus (Sun et al., 2011).
Antigen Affinity Purified from Pooled Serum
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser10,13,14 of human Olig2, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB Brain: 1:1000
Specific for endogenous levels of the ~32 kDa Olig2 phosphorylated at Ser10, Ser 13, and Ser14. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Basic domain helix loop helix protein class B 6 antibody, Basic domain helix loop helix protein class B6 antibody, BHLH B6 antibody, BHLHB 6 antibody, bHLHb6 antibody, bHLHe21 antibody, Class B basic helix-loop-helix protein 6 antibody, Class E basic helix-loop-helix protein 21 antibody, Olig 1 antibody, Olig1 antibody, OLIG1_HUMAN antibody, Oligo 1 antibody, Oligo1 antibody, Oligodendrocyte lineage transcription factor 1 antibody, Oligodendrocyte specific bHLH transcription factor 1 antibody, Oligodendrocyte transcription factor 1 antibody