Immunostaining of HeLa cells showing specific labeling of nuclei using the anti-nuclei antibody (cat. 1590-NUC, 1:100, green).
This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.
Concentrated tissue culture supernatant
Marker for human cells in xenographic research
Human cell homogenate.
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Concentrated tissue ulture supernatant
Concentrated tissue culture supernatent
Specific for endogenous levels of human nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.
Immunofluorescence performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
HNA antibody, Human Nuclear Antigen antibody, HuNu antibody
Glaser, R., Lu, M.M., Narula, N. and Epstein, J.A., 2002. Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation, 106(1), pp.17-19. PMID: 12093763
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