Anti-Nuclei Antibody (235-1)

Name: Anti-Nuclei Antibody
Brand: PhosphoSolutions
SKU: 1590-NUC
Price: 375.0 USD
Availability: InStock

Our Anti-Nuclei primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Nuclei and is concentrated tissue culture supernatant. It is great for use in IHC, ICC.

Immunostaining of HeLa cells showing specific labeling of nuclei using the anti-nuclei antibody (cat. 1590-NUC, 1:100, green).

Species Reactivity

Human

Applications

ICC, IHC

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1590-NUC

Volume: 100 µL

Variant

Price:

$375

Quantity:

Product Details

Target

Nuclei

Target Description

This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.

Format

Concentrated tissue culture supernatant

Clonality

Monoclonal

Clone

235-1

Isotype

IgG1

Applications

ICC, IHC

Host Species

Mouse

Gene Name

Marker for human cells in xenographic research

Antigen

Human cell homogenate.

Species Reactivity

Human

Antibody Registry ID

AB_2492189

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Concentrated tissue ulture supernatant

Buffer

Concentrated tissue culture supernatent

Dilution Ranges

IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of human nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.

Quality Control

Immunofluorescence performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

HNA antibody, Human Nuclear Antigen antibody, HuNu antibody

Product Specific Protocols

Nuclei ICC Protocol: Download

General References

Glaser, R., Lu, M.M., Narula, N. and Epstein, J.A., 2002. Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation, 106(1), pp.17-19. PMID: 12093763

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Anti-Nuclei Antibody (235-1)

SKU: 1590-NUC

Product Details

Product Specific Protocols

General References

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